Parallel determination of glucose metabolism and fractional synthesis rates of individual hepatic proteins during hyperdynamic septic shock

Excerpt

Proteomic platforms allow one to detect changes in the proteome of tissues. They can be combined with the use of stable isotope-labelled tracer amino acids to detect changes in the synthesis or breakdown rate of individual proteins. An ideal complement to these data would be a characterization of the metabolism. We focus on the defense against oxidative stress, which is closely related to energy metabolism. The latter can be assessed using isotope-labelled 1,2,3,4,5,6-¹³C6-glucose and following the metabolic fate of the labelled carbons. We therefore investigated whether an in-vivo septic animal model with labelled glucose would provide useful data about glucose and energy metabolism and whether the same tracer set up could be used to reliably estimate the turnover of individual proteins. …