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BMC Complementary Medicine and Therapies

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Open Access 01-12-2015 | Research article

Paeonolum protects against MPP⁺-induced neurotoxicity in zebrafish and PC12 cells

Authors: Xi-Lin Lu, Yue-Hao Lin, Qi Wu, Feng-Juan Su, Cheng-Hui Ye, Lei Shi, Bai-Xuan He, Fei-Wen Huang, Zhong Pei, Xiao-Li Yao

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

Abstract

Background

Parkinson’s disease (PD) is the second most common neurodegenerative disease, affecting 2% of the population aged over 65 years old. Mitochondrial defects and oxidative stress actively participate in degeneration of dopaminergic (DA) neurons in PD. Paeonolum, a main component isolated from Moutan cortex, has potent antioxidant ability. Here, we have examined the effects of paeonolum against MPP⁺-induced neurotoxicity in zebrafish and PC12 cells.

Methods

The overall viability and neurodegeneration of DA neurons was assessed in ETvmat2:green fluorescent protein (GFP) transgenic zebrafish, in which most monoaminergic neurons are labeled by GFP. Damage to PC12 cells was measured using a cell viability assay and assessment of nuclear morphology. Intracellular reactive oxygen species (ROS) and the level of total GSH were assessed. The mitochondrial cell death pathway including mitochondrial membrane potential, cytochrome C release and caspase-3 activity were also examined in PC12 cells.

Results

Paeonolum protected against MPP⁺-induced DA neurodegeneration and locomotor dysfunction in zebrafish in a concentration-dependent manner. Similar neuroprotection was replicated in the PC12 cellular model of MPP⁺ toxicity. Paeonolum attenuated MPP⁺-induced intracellular ROS accumulation and restored the level of total GSH in PC12 cells. Furthermore, paeonolum significantly inhibited the mitochondrial cell death pathway induced by MPP⁺.

Conclusions

Collectively, the present study demonstrates that paeonolum protects zebrafish and PC12 cells against MPP⁺-induced neurotoxicity.

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Title: Paeonolum protects against MPP+-induced neurotoxicity in zebrafish and PC12 cells
Authors: Xi-Lin Lu
Yue-Hao Lin
Qi Wu
Feng-Juan Su
Cheng-Hui Ye
Lei Shi
Bai-Xuan He
Fei-Wen Huang
Zhong Pei
Xiao-Li Yao
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-015-0661-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Paeonolum protects against MPP⁺-induced neurotoxicity in zebrafish and PC12 cells

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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