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Inflammation Research
Published in: Inflammation Research 12/2013

01-12-2013 | Original Research Paper

Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines

Authors: Chun Wang, Jun Yuan, Hua-xun Wu, Yan Chang, Qing-tong Wang, Yu-jing Wu, Li-hua Liu, Wei Wei

Published in: Inflammation Research | Issue 12/2013

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Abstract

Objective

Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD.

Methods

ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays.

Results

The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL-10 levels were negatively correlated with the inflammation.

Conclusions

The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production.
Appendix
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Metadata
Title
Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines
Authors
Chun Wang
Jun Yuan
Hua-xun Wu
Yan Chang
Qing-tong Wang
Yu-jing Wu
Li-hua Liu
Wei Wei
Publication date
01-12-2013
Publisher
Springer Basel
Published in
Inflammation Research / Issue 12/2013
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-013-0662-8

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