Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Cancer Cell International
Published in: Cancer Cell International 1/2017

Open Access 01-12-2017 | Primary Research

PA-MSHA induces apoptosis and suppresses metastasis by tumor associated macrophages in bladder cancer cells

Authors: Jianjun Liu, Xiaoyu Duan

Published in: Cancer Cell International | Issue 1/2017

Login to get access

Abstract

Background

The aim of the present study was to investigate effects of Pseudomonas aeruginosa-mannose-sensitive hemagglutinin (PA-MSHA) on the inhibition of the proliferation of bladder cancer cell lines and to further define its functional mechanisms.

Methods

A rat model of bladder tumor was induced by intravesical N-methyl-N nitrosourea. The dynamic growth of tumor was measured by whole-body fluorescent imaging system. Morphological analysis was observed by hematoxylin–eosin staining and microscopic examination. The expression of Caspase 3 and E-Ca were detected by immunohistochemistry technique. Macrophages were separated by flow cytometry. The expression of cytokines was measured by qRT-PCR and western blot. Apoptosis ability was conducted by means of annexin V and propidium iodide. The abilities of invasion and migration were determined by transwell migration assay and scratch assay.

Results

PA-MSHA and PA-MSHA + Fisetin groups inhibited the growth of tumor and increased the ratio of M1/M2. For one thing, PA-MSHA suppressed the invasive ability of the bladder tumor cell and promoted bladder tumor cell apoptosis. For another, it facilitated the expression of M1 cytokines and reduced expression of M2 cytokines. Furthermore, treated with PA-MSHA, mouse M1 phagocytosis rates were higher than that of M2 macrophages for bladder cancer lines.

Conclusions

The data revealed that PA-MSHA might promote apoptosis and inhibit proliferation, invasion and migration of mouse bladder cancer cells by inducing M1 polarization.
Literature
1.
Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. A Cancer J Clin. 2013;63(1):11–30.CrossRef
2.
Jemal A, Bray F, Center MM, Ferlay JJ, Ward E, Forman D. Global cancer statistics. Cancer J Clin. 2011;61(2):69–90.CrossRef
3.
Wirth M, Plattner VE, Gabor F. Strategies to improve drug delivery in bladder cancer therapy. Exp Opin Drug Deliv. 2009;6(6):727–44.CrossRef
4.
Mansoor M, Ali S, Fasihuddin Q, Baloch MU. Superficial bladder tumours: recurrence and progression. J Coll Phys Surg Pak. 2011;21(3):157–60.
5.
Pasin E, Josephson DY, Mitra AP, Cote RJ, Stein JP. Superficial bladder cancer: an update on etiology, molecular development, classification, and natural history. Rev Urol. 2008;10(1):31–43.PubMedPubMedCentral
6.
Herr HW. High-risk superficial bladder cancer: transurethral resection alone in selected patients with T1 tumor. J Urol. 1997;160(5):1934.CrossRef
7.
Yun SJ, Kim WJ. Role of the epithelial-mesenchymal transition in bladder cancer: from prognosis to therapeutic target. Korean J Urol. 2013;54(10):645–50.CrossRefPubMedPubMedCentral
8.
Fiorito C, Gontero P. Adverse events after intravesical therapy: are we always ready to recognize and prevent them? Urologia. 2013;80(Suppl 21):27–32.CrossRefPubMed
9.
Griffin JG, Holzbeierlein J. Side effects of perioperative intravesical treatment and treatment strategies for these side effects. Urol Clin N Am. 2013;40(2):197–210.CrossRef
10.
Mead GM, Russell M, Clark P, Harland SJ, Harper PG, Cowan R, Roberts JT, Uscinska BM, Griffiths GO, Parmar MK. A randomized trial comparing methotrexate and vinblastine (MV) with cisplatin, methotrexate and vinblastine (CMV) in advanced transitional cell carcinoma: results and a report on prognostic factors in a Medical Research Council study, MRC Advanced Bladder. Brit J Cancer. 1998;78(8):1067–75.CrossRefPubMedPubMedCentral
11.
Lee N, Ahn B, Jung SB, Kim YG, Kim H, Park WJ. Conformation-dependent antibody response to Pseudomonas aeruginosa outer membrane proteins induced by immunization in humans. FEMS Immunol Med Microbiol. 2000;27(1):79–85.CrossRefPubMed
12.
Porwoll JM, Gebel HM, Rodey GE, Markham RB. In vitro response of human T cells to Pseudomonas aeruginosa. Infect Immun. 1983;40(2):670–4.PubMedPubMedCentral
13.
Cheng GZ, Jia CX, Yang HR. Studies on mechanism of immunization against recurrent urinary tract infection with MSHA—piliated strain vaccine of Pseudomonas aeruginosa. Chin J Microecol. 2000;12:331–3.
14.
Sun W, Hongwen FU, Liu N. Clinical investigation of PA-MSHA strain vaccine on the improvement of immune functions of cancer patients. Chin J Microbiol Immunol. 2000;20(4):373–6.
15.
Li Z, Hao D, Zhang H, Ren L, Yang Y, Li L, Chai J, Zhou X, Fu L. A clinical study on PA-MSHA vaccine used for adjuvant therapy of lymphoma and lung cancer. Hua xi yi ke da xue xue bao. 2000;31(3):334–7.PubMed
16.
Cao Z, Shi L, Li Y, Wang J, Wang D, Wang G, Sun B, Mu L, Yang M, Li H. Pseudomonas aeruginosa: mannose sensitive hemagglutinin inhibits the growth of human hepatocarcinoma cells via mannose-mediated apoptosis. Digest Dis Sci. 2009;54(10):2118–27.CrossRefPubMed
17.
Liu ZB, Hou YF, Di GH, Wu J, Shen ZZ, Shao ZM. PA-MSHA inhibits proliferation and induces apoptosis through the up-regulation and activation of caspases in the human breast cancer cell lines. J Cell Biochem. 2009;108(1):195–206.CrossRefPubMed
18.
Wang J, Wu D, Chen L. Pseudomonas aeruginosa vaccine inhibits the proliferation of human nasopharyngeal cancer cells in vitro. Nan fang yi ke da xue xue bao. 2012;32(4):544–7.PubMed
19.
Wei L, Hua L, Hui C, Feng-Rong YU. Effects of PA-MSHA vaccine on gastric cancer cells in vitro. Chin J Cancer Prev Treat. 2008;15(18):1381–5.
20.
Wang C, Hu Z, Zhu Z, Zhang X, Wei Z, Zhang Y, Hu D, Cai Q. The MSHA strain of Pseudomonas aeruginosa (PA-MSHA) inhibits gastric carcinoma progression by inducing M1 macrophage polarization. Tumor Biol. 2016;37(5):1–9.CrossRef
21.
Zhang M, Gregor H, Kahn SA, Azad TD, Sharareh G, Xu CY, Liu J, Achrol AS, Chase R, Pia S. Anti-CD47 treatment stimulates phagocytosis of glioblastoma by M1 and M2 polarized macrophages and promotes M1 polarized macrophages in vivo. PLoS ONE. 2016;11(4):e0153550.CrossRefPubMedPubMedCentral
22.
Liu ZB, Hou YF, Zhu J, Hu DL, Jin W, Ou ZL, Di GH, Wu J, Shen ZZ, Shao ZM. Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner. Oncogene. 2010;29(20):2996–3009.CrossRefPubMed
23.
Li J, Qu W, Cheng Y, Sun Y, Jiang Y, Zou T, Wang Z, Xu Y, Zhao H. The inhibitory effect of intravesical fisetin against bladder cancer by induction of p53 and down-regulation of NF-kappa B pathways in a rat bladder carcinogenesis model. Basic Clin Pharmacol Toxicol. 2014;115(4):321–9.CrossRefPubMed
24.
Li J, Wu M, Qu W, Cheng Y, Yu Y, Zou T, Jiang Y, Zhao H. Preventive efects of isetin on bladder cancer in rats and its mechanisms. J Modern Oncol. 2014;22(07):1514–9.
25.
Tang W, He Y, Zhou S, Ma Y, Liu G. A novel Bifidobacterium infantis-mediated TK/GCV suicide gene therapy system exhibits antitumor activity in a rat model of bladder cancer. J Exp Clin Cancer Res. 2009;28(1):1–7.CrossRef
26.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-∆∆C(T)) method. Methods. 2001;25(4):402–8.CrossRefPubMed
27.
Xu WH, Liu ZB, Hou YF, Hong Q, Hu DL, Shao ZM. Inhibition of autophagy enhances the cytotoxic effect of PA-MSHA in breast cancer. BMC Cancer. 2014;14(1):1–9.CrossRefPubMedPubMedCentral
28.
Zhu YP, Bian XJ, Ye DW, Yao XD, Zhang SL, Dai B, Shen YJ. Pseudomonas aeruginosa-mannose-sensitive hemagglutinin inhibits proliferation and induces apoptosis in a caspase-dependent manner in human bladder cancer cell lines. Oncol Lett. 2013;5(4):1357–62.PubMedPubMedCentral
29.
30.
Richards JO, Karki S, Lazar GA, Chen H, Wei D, Desjarlais JR. Optimization of antibody binding to FcγRIIa enhances macrophage phagocytosis of tumor cells. Mol Cancer Ther. 2008;7(8):2517–27.CrossRefPubMed
31.
Van Ginderachter JA, Movahedi K, Ghassabeh GH, Meerschaut S, Beschin A, Raes G, De Baetselier P. Classical and alternative activation of mononuclear phagocytes: picking the best of both worlds for tumor promotion. Immunobiology. 2006;211(6–8):487–501.CrossRefPubMed
32.
Barresi V, Branca G, Ieni A, Rigoli L, Tuccari G, Caruso RA. Phagocytosis (cannibalism) of apoptotic neutrophils by tumor cells in gastric micropapillary carcinomas. World J Gastroenterol. 2015;21(18):5548–54.CrossRefPubMedPubMedCentral
33.
Biswas SK, Lewis CE. NF-κB as a central regulator of macrophage function in tumors. J Leukocyte Biol. 2010;88(5):877–84.CrossRefPubMed
34.
Mancino A, Lawrence T. Nuclear factor-kappaB and tumor-associated macrophages. Clin Cancer Res. 2010;16(3):784–9.CrossRefPubMed
Metadata
Title
PA-MSHA induces apoptosis and suppresses metastasis by tumor associated macrophages in bladder cancer cells
Authors
Jianjun Liu
Xiaoyu Duan
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2017
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-017-0445-3

Other articles of this Issue 1/2017

Cancer Cell International 1/2017 Go to the issue

Erratum

Erratum to: In vivo enhancement of the MAGE-specific cellular immune response by a recombinant MAGE1-MAGE3-TBHSP70 tumor vaccine

Primary Research

The human, F-actin-based cytoskeleton as a mutagen sensor

Primary research

The prognostic role of the cancer stem cell marker CD44 in ovarian cancer: a meta-analysis

Primary Research

The impact of γ-irradiation on the induction of bystander killing by genetically engineered ovarian tumor cells: implications for clinical use

Primary research

HLBT-100: a highly potent anti-cancer flavanone from Tillandsia recurvata (L.) L.

Primary research

Establishment and characterization of a novel highly aggressive gallbladder cancer cell line, TJ-GBC2
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits