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Clinical Oral Investigations
Published in: Clinical Oral Investigations 6/2014

01-07-2014 | Short Communication

Oxidative stress is responsible for genotoxicity of camphorquinone in primary human gingival fibroblasts

Authors: Miriam Wessels, Gabriele Leyhausen, Joachim Volk, Werner Geurtsen

Published in: Clinical Oral Investigations | Issue 6/2014

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Abstract

Objectives

The photoinitiator camphorquinone (CQ), used in dental restorative materials, was found to be cytotoxic in cell cultures. Previously, we have shown that CQ induces alkali labile sites and DNA strand breaks in human gingival fibroblasts (HGF) associated with an increase of intracellular reactive oxygen species (ROS). Therefore, the objective of our study was to evaluate if DNA damage in HGF cells is caused by the generation of ROS.

Material and methods

HGF cells were treated with different concentrations (0.5–2.5 mM) of CQ. The cell viability was assessed using propidium iodide (PI) assay. Oxidative DNA damage was evaluated by an enzyme-modified comet assay using human 8-hydroxyguanine DNA-glycosylase 1 (hOGG1), which converts oxidized 7,8-dihydro-8-oxoguanine (8-oxoguanine) into DNA strand breaks and functions as a marker for oxidative modified DNA.

Results

The results showed that CQ induced DNA damage in HGF cells without cytotoxic effects for the chosen treatment time. CQ treatment led to the generation of 8-oxoguanine in DNA, which can be shown by a significant increase in tail moment after CQ treatment by the enzyme-modified comet assay.

Conclusion

It may be concluded that DNA damage due to CQ is caused by oxidative stress in gingival fibroblasts.

Clinical relevance

A more detailed insight into genotoxic mechanisms in oral cells can be of great importance for a better understanding of the biocompatibility of CQ.
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Metadata
Title
Oxidative stress is responsible for genotoxicity of camphorquinone in primary human gingival fibroblasts
Authors
Miriam Wessels
Gabriele Leyhausen
Joachim Volk
Werner Geurtsen
Publication date
01-07-2014
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 6/2014
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-013-1178-x

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