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BMC Cancer
Published in: BMC Cancer 1/2006

Open Access 01-12-2006 | Research article

Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer

Authors: Hui Wang, Li-Na Zhao, Kai-Zong Li, Rui Ling, Xiao-Jun Li, Ling Wang

Published in: BMC Cancer | Issue 1/2006

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Abstract

Background

Ribosomal proteins are the components of ribosome, which also exhibit various secondary functions in DNA repair, apoptosis, drug resistance and proliferation. In our previous study of microarray, ribosomal protein L15 (RPL15) was identified as an upregulated gene in gastric cancer.

Methods

We investigated the expression of ribosomal protein L15 in gastric cancer and the effect of RPL15 on proliferation of gastric cancer.

Results

It was found that the expression of RPL15 was markedly up-regulated in gastric cancer tissues. RPL15 was also highly expressed in gastric cancer cell lines AGS, MKN45, MKN28, SGC7901 and KATOIII. Inhibition of RPL15 expression by siRNA vector transfection suppressed the growth of SGC7901 cells significantly, which was independent of the expression of Cyclin D1 and B1. Down-regulation of RPL15 expression inhibited SGC7901 cell growth in soft agar and its tumorigenicity in nude mice.

Conclusion

RPL15 promotes cell proliferation and may be a potential target for anticancer therapy of gastric cancer.
Appendix
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Literature
1.
Plummer M, Franceschi S, Munoz N: Epidemiology of gastric cancer. IARC Sci Publ. 2004, 157: 311-326.PubMed
2.
3.
Tahara E: Genetic pathways of two types of gastric cancer. IARC Sci Publ. 2004, 157: 327-349.PubMed
4.
El-Rifai W, Powell SM: Molecular biology of gastric cancer. Semin Radiat Oncol. 2002, 12: 128-140. 10.1053/srao.2002.30815.CrossRefPubMed
5.
Pan Y, Bi F, Liu N, Xue Y, Yao X, Zheng Y, Fan D: Expression of seven main Rho family members in gastric carcinoma. Biochem Biophys Res Commun. 2004, 315: 686-91. 10.1016/j.bbrc.2004.01.108.CrossRefPubMed
6.
Leung SY, Yuen ST, Chu KM, Mathy JA, Li R, Chan AS, Law S, Wong J, Chen X, So S: Expression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer. Gastroenterology. 2004, 127: 457-469. 10.1053/j.gastro.2004.05.031.CrossRefPubMed
7.
Meireles SI, Cristo EB, Carvalho AF, Hirata R, Pelosof A, Gomes LI, Martins WK, Begnami MD, Zitron C, Montagnini AL, Soares FA, Neves EJ, Reis LF: Molecular classifiers for gastric cancer and nonmalignant diseases of the gastric mucosa. Cancer Res. 2004, 64: 1255-1265. 10.1158/0008-5472.CAN-03-1850.CrossRefPubMed
8.
Kim B, Bang S, Lee S, Kim S, Jung Y, Lee C, Choi K, Lee SG, Lee K, Lee Y, Kim SS, Yeom YI, Kim YS, Yoo HS, Song K, Lee I: Expression profiling and subtype-specific expression of stomach cancer. Cancer Res. 2003, 63: 8248-8255.PubMed
9.
Wang Q, Yang C, Zhou J, Wang X, Wu M, Liu Z.: Cloning and characterization of full-length human ribosomal protein L15 cDNA which was overexpressed in esophageal cancer. Gene. 2001, 263: 205-209. 10.1016/S0378-1119(00)00570-9.CrossRefPubMed
10.
Maaser K, Daubler P, Barthel B, Heine B, von Lampe B, Stein H, Hoffmeister B, Scherer H, Scherubl H: Oesophageal squamous cell neoplasia in head and neck cancer patients: upregulation of COX-2 during carcinogenesis. Br J Cancer. 2003, 88: 1217-1222. 10.1038/sj.bjc.6600865.CrossRefPubMedPubMedCentral
11.
Liu N, Bi F, Pan Y, Sun L, Xue Y, Shi Y, Yao X, Zheng Y, Fan D: Reversal of the malignant phenotype of gastric cancer cells by inhibition of RhoA expression and activity. Clin Cancer Res. 2004, 10: 6239-6247. 10.1158/1078-0432.CCR-04-0242.CrossRefPubMed
12.
Clemens MJ: Targets and mechanisms for the regulation of translation in malignant transformation. Oncogene. 2004, 23: 3180-3188. 10.1038/sj.onc.1207544.CrossRefPubMed
13.
Stoneley M, Willis AE: Aberrant regulation of translation initiation in tumorigenesis. Curr Mol Med. 2003, 3: 597-603. 10.2174/1566524033479474.CrossRefPubMed
14.
Frank J: The ribosome – a macromolecular machine par excellence. Chem Biol. 2000, 7: R133-141. 10.1016/S1074-5521(00)00127-7.CrossRefPubMed
15.
Kasai H, Nadano D, Hidaka E, Higuchi K, Kawakubo M, Sato TA, Nakayama J: Differential expression of ribosomal proteins in human normal and neoplastic colorectum. J Histochem Cytochem. 2003, 51: 567-574.CrossRefPubMed
16.
Kim JH, You KR, Kim IH, Cho BH, Kim CY, Kim DG: Over-expression of the ribosomal protein L36a gene is associated with cellular proliferation in hepatocellular carcinoma. Hepatology. 2004, 39: 129-138. 10.1002/hep.20017.CrossRefPubMed
17.
Kondoh N, Shuda M, Tanaka K, Wakatsuki T, Hada A, Yamamoto M: Enhanced expression of S8, L12, L23a, L27 and L30 ribosomal protein mRNAs in human hepatocellular carcinoma. Anticancer Res. 2001, 21: 2429-2433.PubMed
18.
Shriver SP, Shriver MD, Tirpak DL, Bloch LM, Hunt JD, Ferrell RE, Siegfried JM: Trinucleotide repeat length variation in the human ribosomal protein L14 gene (RPL14): localization to 3p21.3 and loss of heterozygosity in lung and oral cancers. Mutat Res. 1998, 406: 9-23.PubMed
19.
Chen FW, Ioannou YA: Ribosomal proteins in cell proliferation and apoptosis. Int Rev Immunol. 1999, 18: 429-448.CrossRefPubMed
20.
Khanna N, Sen S, Sharma H, Singh N: S29 ribosomal protein induces apoptosis in H520 cells and sensitizes them to chemotherapy. Biochem Biophys Res Commun. 2003, 304: 26-35. 10.1016/S0006-291X(03)00532-1.CrossRefPubMed
21.
Chen FW, Davies JP, Ioannou YA: Differential gene expression in apoptosis: identification of ribosomal protein 23K, a cell proliferation inhibitor. Mol Genet Metab. 1998, 64: 271-282. 10.1006/mgme.1998.2718.CrossRefPubMed
Metadata
Title
Overexpression of ribosomal protein L15 is associated with cell proliferation in gastric cancer
Authors
Hui Wang
Li-Na Zhao
Kai-Zong Li
Rui Ling
Xiao-Jun Li
Ling Wang
Publication date
01-12-2006
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2006
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-6-91

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