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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2011 | Research

Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway

Authors: Xiaoman Xu, Yi Zhang, Dan Qu, Tingshu Jiang, Shengqi Li

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2011

Abstract

Background

To explore the effects of Osthole on the proliferation, cell cycle and apoptosis of human lung cancer A549 cells.

Methods

Human lung cancer A549 cells were treated with Osthole at different concentrations. Cell proliferation was measured using the MTT assay. Cell cycle was evaluated using DNA flow cytometry analysis. Induction of apoptosis was determined by flow cytometry and fluorescent microscopy. The expressions of Cyclin B1, p-Cdc2, Bcl-2, Bax, t-Akt and p-Akt were evaluated by Western blotting.

Results

Osthole inhibited the growth of human lung cancer A549 cells by inducing G2/M arrest and apoptosis. Western blotting demonstrated that Osthole down-regulated the expressions of Cyclin B1, p-Cdc2 and Bcl-2 and up-regulated the expressions of Bax in A549 cells. Inhibition of PI3K/Akt signaling pathway was also observed after treating A549 cells with Osthole.

Conclusions

Our findings suggest that Osthole may have a therapeutic application in the treatment of human lung cancer.

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Title: Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway
Authors: Xiaoman Xu
Yi Zhang
Dan Qu
Tingshu Jiang
Shengqi Li
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2011
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/1756-9966-30-33

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Abstract

Background

Methods

Results

Conclusions

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