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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2024 | Osteoporosis | Research article

circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis

Authors: Lian Tang, Lin Yuan, Jiyuan Yan, Jianhua Ge, Zhi Lian, Zhong Li

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2024

Abstract

Objective

Osteoporosis is the imbalance in bone homeostasis between osteoblasts and osteoclasts. In this study, we investigated the effects of the circ_0029463/miR-134-5p/Rab27a axis on RANKL-induced osteoclast differentiation.

Methods

RT-qPCR and western blotting were used to detect the expression of circ_0029463, miR-134-5p, and Rab27a in tissues from patients with osteoporosis and in RANKL-induced osteoclasts. Osteoclast differentiation was verified by TRAP staining. Osteoclast biomarkers, including NFATc1, TRAP, and CTSK, were measured. The target and regulatory relationships between circ_0029463, miR-134-5p, and the Rab27a axis were verified using RIP, dual-luciferase reporter gene, and RNA pull-down assays.

Results

Elevated expression of circ_0029463 and Rab27a and decreased miR-134-5p expression were observed in the tissues of patients with osteoporosis, and a similar expression pattern was observed in RANKL-induced osteoclasts. Suppression of circ_0029463 expression or miR-134-5p overexpression curbed RANKL-induced osteoclast differentiation, whereas such an effect was abolished by Rab27 overexpression. circ_0029463 sponges miR-134-5p to induce Rab27a expression.

Conclusion

circ_0029463 sponges miR-134-5p to abolish its suppressive effect of miR-134-5p on Rab27a expression, thereby promoting osteoclast differentiation.

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Title: circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis
Authors: Lian Tang
Lin Yuan
Jiyuan Yan
Jianhua Ge
Zhi Lian
Zhong Li
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Osteoporosis
Osteoporosis
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2024
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-024-04610-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

circ_0029463 promotes osteoclast differentiation by mediating miR-134-5p/Rab27a axis

Abstract

Objective

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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