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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2015

Open Access 01-12-2015 | Research article

Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy

Authors: Amanda Maria Sena Reis, Lorena Gabriela Rocha Ribeiro, Natália de Melo Ocarino, Alfredo Miranda Goes, Rogéria Serakides

Published in: BMC Musculoskeletal Disorders | Issue 1/2015

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Abstract

Background

Caffeine is an active alkaloid that can cause damage to bones in formation during prenatal life into adulthood. This compound can pass across the placenta and into the mother’s milk, causing a reduction in bone formation, growth and mass. The objective of this study was to examine the osteogenic potential of osteoblasts extracted from neonatal rats born to mothers treated with caffeine throughout pregnancy.

Methods

Twenty-four adult Wistar rats were randomly divided into four groups, consisting of one control group and three groups that were treated with 25, 50, or 100 mg/kg of caffeine by an oral-gastric probe throughout the duration of the experimental period (pregnancy). At birth, three puppies from each dam in each group were euthanized, and osteoblasts were extracted from the calvaria of these pups for in vitro testing.

Results

The osteoblasts extracted from the pups of rats that received 50 mg/kg caffeine during pregnancy exhibited increased expression of osteocalcin, osteopontin, sialoprotein, runx-2, alkaline phosphatase and type I collagen transcripts, resulting in increased synthesis of mineralization nodules.

Conclusions

Neonates from rats treated with 50 mg/kg caffeine during pregnancy contained osteoblasts with a higher osteogenic potential characterized by increased expression of osteocalcin, osteopontin, sialoprotein, runx-2, alkaline phosphatase and type I collagen and increased synthesis of mineralization nodules.
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Metadata
Title
Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy
Authors
Amanda Maria Sena Reis
Lorena Gabriela Rocha Ribeiro
Natália de Melo Ocarino
Alfredo Miranda Goes
Rogéria Serakides
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2015
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-015-0467-8

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