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European Journal of Clinical Microbiology & Infectious Diseases

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01-11-2008 | Article

Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil

Authors: E. R. L. Freitas, C. M. A. Soares, F. S. Fiaccadori, M. Souza, J. A. Parente, P. S. S. Costa, D. D. P. Cardoso

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 11/2008

Abstract

Group A rotaviruses (RVA) are the main causing agents of acute gastroenteritis worldwide, having a great impact on childhood mortality in developing countries. The objective of this study was to identify RVA-positive fecal samples with mixed P genotypes by hemi-nested reverse transcriptase-polymerase chain reaction (RT-PCR), followed by sequencing confirmation. Our results showed that, from the 81 RVA-positive samples, 25 were positive for more than one P genotype by hemi-nested RT-PCR. Of these 25 samples, 12 (48%) had their mixed P genotypes confirmed by sequencing and, from these, 10 were identified as P[6]P[8], one as P[4]P[6], and one as P[4]P[6]P[8]. Our results confirm the occurrence of RVA mixed infections among children in Brazil and reinforce the importance of the constant monitoring of RVA circulating strains for the efficacy of control/prevention against these agents.

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Title: Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil
Authors: E. R. L. Freitas
C. M. A. Soares
F. S. Fiaccadori
M. Souza
J. A. Parente
P. S. S. Costa
D. D. P. Cardoso
Publication date: 01-11-2008
Publisher: Springer-Verlag
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 11/2008
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-008-0542-2

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