Top

BMC Complementary Medicine and Therapies

Published in:

01-12-2020 | Obesity | Research article

Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion

Authors: Cécile Héliès-Toussaint, Edwin Fouché, Nathalie Naud, Florence Blas-Y-Estrada, Maria del Socorro Santos-Diaz, Anne Nègre-Salvayre, Ana Paulina Barba de la Rosa, Françoise Guéraud

Published in: BMC Complementary Medicine and Therapies | Issue 1/2020

Abstract

Background

Obesity is a major public health concern worldwide. A sedentary life and a nutritional transition to processed foods and high-calorie diets are contributing factors to obesity. The demand for nutraceutical foods, such as herbal weight-loss products, which offer the potential to counteract obesity, has consequently increased. We hypothesised that Opuntia cladodes consumption could assist weight management in an obesity prevention context.

Methods

This study was designed to explore the anti-adipogenic effects of lyophilised Opuntia cladode powders (OCP) in an in vitro cellular model for adipocyte differentiation and an in vivo high-fat-diet (HFD)-induced obesity rat model. Two OCP were tested, one from wild species O. streptacantha and the second from the most known species O. ficus-indica.

Results

Pre-adipocytes 3 T3-F442A were treated by OCP during the differentiation process by insulin. OCP treatment impaired the differentiation in adipocytes, as supported by the decreased triglyceride content and a low glucose uptake, which remained comparable to that observed in undifferentiated controls, suggesting that an anti-adipogenic effect was exerted by OCP. Sprague–Dawley rats were fed with a normal or HFD, supplemented or not with OCP for 8 weeks. OCP treatment slightly reduced body weight gain, liver and abdominal fat weights, improved some obesity-related metabolic parameters and increased triglyceride excretion in the faeces. Taken together, these results showed that OCP might contribute to reduce adipogenesis and fat storage in a HFD context, notably by promoting the faecal excretion of fats.

Conclusions

Opuntia cladodes may be used as a dietary supplement or potential therapeutic agent in diet-based therapies for weight management to prevent obesity.

Graphical abstract

WHO | Obesity and overweight. WHO n.d. https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight. Accessed 26 Oct 2017.

World Cancer Research Fund/American Institute for Cancer Research. Continuous Update Project Expert Report 2018. Diet, nutrition and physical activity: Energy balance and body fatness. 2018. Available at https://www.wcrf.org/dietandcancer/energy-balance-body-fatness.

Avila-Nava A, Noriega LG, Tovar AR, Granados O, Perez-Cruz C, Pedraza-Chaverri J, et al. Food combination based on a pre-hispanic Mexican diet decreases metabolic and cognitive abnormalities and gut microbiota dysbiosis caused by a sucrose-enriched high-fat diet in rats. MolNutr Food Res. 2017;61. https://doi.org/10.1002/mnfr.201501023.CrossRef

Rtveladze K, Marsh T, Barquera S, Romero LMS, Levy D, Melendez G, et al. Obesity prevalence in Mexico: impact on health and economic burden. Public Health Nutr. 2014;17:233–9. https://doi.org/10.1017/S1368980013000086.CrossRefPubMed

Hasani-Ranjbar S, Jouyandeh Z, Abdollahi M. A systematic review of anti-obesity medicinal plants - an update. J Diabetes MetabDisord. 2013;12:28. https://doi.org/10.1186/2251-6581-12-28.CrossRef

del Socorro Santos Díaz M, Barba de la Rosa A-P, Héliès-Toussaint C, Guéraud F, Nègre-Salvayre A. Opuntia spp.: Characterization and Benefits in Chronic Diseases. Oxidative Med Cell Longev. 2017. https://doi.org/10.1155/2017/8634249.CrossRef

Ojulari OV, Lee SG, Nam J-O. Beneficial Effects of Natural Bioactive Compounds from Hibiscus sabdariffa L on Obesity. Molecules. 2019;24:210. https://doi.org/10.3390/molecules24010210.CrossRefPubMedCentral

Yang H-W, Fernando KHN, Oh J-Y, Li X, Jeon Y-J, Ryu B. Anti-obesity and anti-diabetic effects of Ishigeokamurae. Mar Drugs. 2019;17:202. https://doi.org/10.3390/md17040202.CrossRefPubMedCentral

Uebelhack R, Busch R, Alt F, Beah Z-M, Chong P-W. Effects of Cactus Fiber on the excretion of dietary fat in healthy subjects: a double blind, randomized, placebo-controlled, crossover clinical investigation. Curr Ther Res. 2014;76:39–44. https://doi.org/10.1016/j.curtheres.2014.02.001.CrossRefPubMed

10.

Grube B, Chong P-W, Lau K-Z, Orzechowski H-D. A natural Fiber complex reduces body weight in the overweight and obese: a double-blind, randomized, Placebo-Controlled Study. Obesity. 2012. https://doi.org/10.1038/oby.2012.165.

11.

El-Mostafa K, Kharrassi Y, Badreddine A, Andreoletti P, Vamecq J, Kebbaj M, et al. Nopal Cactus (Opuntiaficus-indica) as a source of bioactive compounds for nutrition, health and disease. Molecules. 2014;19:14879–901. https://doi.org/10.3390/molecules190914879.CrossRefPubMedPubMedCentral

12.

Astello-García MG, Cervantes I, Nair V. Santos-Díaz M del S, Reyes-Agüero a, Guéraud F, et al. chemical composition and phenolic compounds profile of cladodes from Opuntia spp. cultivars with different domestication gradient. J Food Compos Anal. 2015;43:119–30. https://doi.org/10.1016/j.jfca.2015.04.016.CrossRef

13.

Keller J, Camaré C, Bernis C, Astello-García M, de la Rosa A-PB, Rossignol M, et al. Antiatherogenic and antitumoral properties of Opuntia cladodes: inhibition of low density lipoprotein oxidation by vascular cells, and protection against the cytotoxicity of lipid oxidation product 4-hydroxynonenal in a colorectal cancer cellular model. J Physiol Biochem. 2015;71:577–87. https://doi.org/10.1007/s13105-015-0408-x.CrossRefPubMed

14.

Garoby-Salom S, Guéraud F, Camaré C, de la Rosa A-PB, Rossignol M, del SS DM, et al. Dietary cladode powder from wild type and domesticated Opuntia species reduces atherogenesis in apoEknock-out mice. J Physiol Biochem. 2016;72:59–70.CrossRef

15.

Rodríguez-Rodríguez C, Torres N, Gutiérrez-Uribe JA, Noriega LG, Torre-Villalvazo I, Leal-Díaz AM, et al. The effect of isorhamnetin glycosides extracted from Opuntiaficus-indica in a mouse model of diet induced obesity. Food Funct. 2015;6:805–15. https://doi.org/10.1039/C4FO01092B.CrossRefPubMed

16.

Lee Y-J, Choi H-S, Seo M-J, Jeon H-J, Kim K-J, Lee B-Y. Kaempferol suppresses lipid accumulation by inhibiting early adipogenesis in 3T3-L1 cells and zebrafish. Food Funct. 2015;6:2824–33. https://doi.org/10.1039/C5FO00481K.CrossRefPubMed

17.

Sánchez-Tapia M, Aguilar-López M, Pérez-Cruz C, Pichardo-Ontiveros E, Wang M, Donovan SM, et al. Nopal (Opuntiaficusindica) protects from metabolic endotoxemia by modifying gut microbiota in obese rats fed high fat/sucrose diet. Sci Rep. 2017;7. https://doi.org/10.1038/s41598-017-05096-4.

18.

Morán-Ramos S, Avila-Nava A, Tovar AR, Pedraza-Chaverri J, López-Romero P, Torres N. Opuntiaficusindica (nopal) attenuates hepatic steatosis and oxidative stress in obese Zucker (fa/fa) rats. J Nutr. 2012;142:1956–63. https://doi.org/10.3945/jn.112.165563.CrossRefPubMed

19.

Zang Y, Zhang L, Igarashi K, Yu C. The anti-obesity and anti-diabetic effects of kaempferol glycosides from unripe soybean leaves in high-fat-diet mice. Food Funct. 2015;6:834–41. https://doi.org/10.1039/c4fo00844h.CrossRefPubMed

20.

Bounihi A, Bitam A, Bouazza A, Yargui L, Koceir EA. Fruit vinegars attenuate cardiac injury via anti-inflammatory and anti-adiposity actions in high-fat diet-induced obese rats. Pharm Biol. 2017;55:43–52. https://doi.org/10.1080/13880209.2016.1226369.CrossRefPubMed

21.

Lee SG, Lee YJ, Jang M-H, Kwon TR, Nam J-O. Panax ginseng leaf extracts exert anti-obesity effects in high-fat diet-induced obese rats. Nutrients. 2017;9:999. https://doi.org/10.3390/nu9090999.CrossRefPubMedCentral

22.

Moon J, Do H-J, Kim OY, Shin M-J. Antiobesity effects of quercetin-rich onion peel extract on the differentiation of 3T3-L1 preadipocytes and the adipogenesis in high fat-fed rats. Food Chem Toxicol. 2013;58:347–54. https://doi.org/10.1016/j.fct.2013.05.006.CrossRefPubMed

23.

Ramírez-Tobías HM, Reyes-Agüero JA, Pinos-Rodríguez JM, Aguirre-Rivera JR, Ramírez-Tobías HM, Reyes-Agüero JA, et al. Efecto de la especie y madurez sobre el contenido de nutrientes de cladodios de nopal. Agrociencia. 2007;41:619–26.

24.

Kim JH, Park JM, Kim E-K, Lee JO, Lee SK, Jung JH, et al. Curcumin stimulates glucose uptake through AMPK-p38 MAPK pathways in L6 myotube cells. J Cell Physiol. 2010:n/a-n/a. https://doi.org/10.1002/jcp.22093.

25.

Héliès-Toussaint C, Peyre L, Costanzo C, Chagnon M-C, Rahmani R. Is bisphenol S a safe substitute for bisphenol a in terms of metabolic function? An in vitro study. Toxicol Appl Pharmacol. 2014;280:224–35. https://doi.org/10.1016/j.taap.2014.07.025.CrossRefPubMed

26.

Rosen ED, Spiegelman BM. Adipocytes as regulators of energy balance and glucose homeostasis. Nature. 2006;444:847–53. https://doi.org/10.1038/nature05483.CrossRefPubMedPubMedCentral

27.

Lai C-S, Liao S-N, Tsai M-L, Kalyanam N, Majeed M, Majeed A, et al. Calebin-a inhibits adipogenesis and hepatic steatosis in high-fat diet-induced obesity via activation of AMPK signaling. Mol Nutr Food Res. 2015;59:1883–95. https://doi.org/10.1002/mnfr.201400809.CrossRefPubMed

28.

Jensen VS, Hvid H, Damgaard J, Nygaard H, Ingvorsen C, Wulff EM, et al. Dietary fat stimulates development of NAFLD more potently than dietary fructose in Sprague-Dawley rats. DiabetolMetabSyndr. 2018;10:4. https://doi.org/10.1186/s13098-018-0307-8.CrossRef

29.

Alonso-Castro AJ, Salazar-Olivo LA. The anti-diabetic properties of Guazumaulmifolia lam are mediated by the stimulation of glucose uptake in normal and diabetic adipocytes without inducing adipogenesis. J Ethnopharmacol. 2008;118:252–6. https://doi.org/10.1016/j.jep.2008.04.007.CrossRefPubMed

30.

Alonso-Castro AJ, Zapata-Bustos R, Romo-Yañez J, Camarillo-Ledesma P, Gómez-Sánchez M, Salazar-Olivo LA. The antidiabetic plants Tecomastans (L.) Juss. exKunth (Bignoniaceae) and Teucriumcubense Jacq (Lamiaceae) induce the incorporation of glucose in insulin-sensitive and insulin-resistant murine and human adipocytes. J Ethnopharmacol. 2010;127:1–6. https://doi.org/10.1016/j.jep.2009.09.060.CrossRefPubMed

31.

Song Y, Park HJ, Kang SN, Jang S-H, Lee S-J, Ko Y-G, et al. Blueberry Peel extracts inhibit Adipogenesis in 3T3-L1 cells and reduce high-fat diet-induced obesity. PLoS One. 2013;8:e69925. https://doi.org/10.1371/journal.pone.0069925.CrossRefPubMedPubMedCentral

32.

Son Y, Nam J-S, Jang M-K, Jung I-A, Cho S-I, Jung M-H. Antiobesity activity of Vignanakashimae extract in high-fat diet-induced obesity. Biosci Biotechnol Biochem. 2013;77:332–8. https://doi.org/10.1271/bbb.120755.CrossRefPubMed

33.

Yu Z-W, Burén J, Enerbäck S, Nilsson E, Samuelsson L, Eriksson JW. Insulin can enhance GLUT4 gene expression in 3T3-F442A cells and this effect is mimicked by vanadate but counteracted by cAMP and high glucose – potential implications for insulin resistance. Biochimica et BiophysicaActa (BBA) - Molecular Basis of Disease. 2001;1535:174–85. https://doi.org/10.1016/S0925-4439(00)00097-1.CrossRef

34.

Carpéné C, Les F, Cásedas G, Peiro C, Fontaine J, Chaplin A, et al. Resveratrol anti-obesity effects: rapid inhibition of adipocyte glucose utilization. Antioxidants. 2019;8:74. https://doi.org/10.3390/antiox8030074.CrossRefPubMedCentral

35.

Hoek-van den Hil EF, van Schothorst EM, van der Stelt I, Swarts HJM, Venema D, Sailer M, et al. Quercetin decreases high-fat diet induced body weight gain and accumulation of hepatic and circulating lipids in mice. Genes Nutr. 2014:9. https://doi.org/10.1007/s12263-014-0418-2.

36.

Lee J, Jung E, Lee J, Kim S, Huh S, Kim Y, et al. Isorhamnetin represses Adipogenesis in 3T3-L1 cells. Obesity. 2009;17:226–32. https://doi.org/10.1038/oby.2008.472.CrossRefPubMed

37.

Barr VA, Malide D, Zarnowski MJ, Taylor SI, Cushman SW. Insulin stimulates both leptin secretion and production by rat white adipose tissue. Endocrinology. 1997;138:4463–72.CrossRef

38.

Fu Y, Luo N, Klein RL, Garvey WT. Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation. J Lipid Res. 2005;46:1369–79. https://doi.org/10.1194/jlr.%20M400373-JLR200.CrossRefPubMed

39.

Kim M-H, Kang K-S, Lee Y-S. The inhibitory effect of genistein on hepatic steatosis is linked to visceral adipocyte metabolism in mice with diet-induced non-alcoholic fatty liver disease. Br J Nutr. 2010;104:1333–42. https://doi.org/10.1017/S0007114510002266.CrossRefPubMed

40.

Anderson N, Borlak J. Molecular mechanisms and therapeutic targets in Steatosis and Steatohepatitis. Pharmacol Rev. 2008;60:311–57. https://doi.org/10.1124/pr.108.00001.CrossRefPubMed

41.

Chong P-W, Lau K-Z, Gruenwald J, Uebelhack R. A review of the efficacy and safety of Litramine IQP-G-002AS, an Opuntiaficus-indica derived Fiber for weight management. Evid Based Complement Alternat Med. 2014;2014:1–6. https://doi.org/10.1155/2014/943713.CrossRef

Title: Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion
Authors: Cécile Héliès-Toussaint
Edwin Fouché
Nathalie Naud
Florence Blas-Y-Estrada
Maria del Socorro Santos-Diaz
Anne Nègre-Salvayre
Ana Paulina Barba de la Rosa
Françoise Guéraud
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Obesity
Obesity
Insulins
Insulins
Published in: BMC Complementary Medicine and Therapies / Issue 1/2020
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-020-2824-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Opuntia cladode powders inhibit adipogenesis in 3 T3-F442A adipocytes and a high-fat-diet rat model by modifying metabolic parameters and favouring faecal fat excretion

Abstract

Background

Methods

Results

Conclusions

Graphical abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Graphical abstract

Please log in to get access to this content

Other articles of this Issue 1/2020

Study on the mechanisms of compound Kushen injection for the treatment of gastric cancer based on network pharmacology

PAMs inhibits monoamine oxidase a activity and reduces glioma tumor growth, a potential adjuvant treatment for glioma

Soyasaponins reduce inflammation by downregulating MyD88 expression and suppressing the recruitments of TLR4 and MyD88 into lipid rafts

Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways

The effect of aged garlic extract on the atherosclerotic process – a randomized double-blind placebo-controlled trial

Xanthine oxidase inhibitory activity of a new isocoumarin obtained from Marantodes pumilum var. pumila leaves