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Journal of Cardiothoracic Surgery

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Open Access 01-12-2021 | NSCLC | Research article

CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation

Authors: Juan Chen, Jiang Gong

Published in: Journal of Cardiothoracic Surgery | Issue 1/2021

Abstract

Background

CircMMP11 is a characterized circRNA with oncogenic function in breast cancer. In this study, we explored the involvement of circMMP11 in non-small cell lung cancer (NSCLC).

Methods

Paired cancer and non-cancer tissues were collected from 66 NSCLC patients, and the expression of circMMP11 and miR-143 in these tissues were detected using RT-qPCRs. Overexpression levels of circMMP11 and miR-143 were performed by transfection, and their crosstalk was analyzed by RT-qPCRs. The effect of circMMP11 overexpression on miR-143 methylation was analyzed by methylation-specific PCR. CCK-8 assay was performed to analyze the roles of miR-143 and circMMP11 in regulating NSCLC cell proliferation.

Results

We found that circMMP11 was overexpressed in NSCLC and predicted patients’ poor survival. Moreover, a close correlation between circMMP11 and miR143 was observed. In NSCLC cells, circMMP11 overexpression reduced miR-143 expression and increased miR-143 methylation. CCK-8 assay analysis showed that miR-143 reversed the enhancing effects of circMMP11 overexpression on cell proliferation.

Conclusions

CircMMP11 is overexpressed in NSCLC and predicts poor survival. In addition, circMMP11 may downregulate miR-143 through methylation to suppress cell proliferation.

Hirsch FR, Scagliotti GV, Mulshine JL, Kwon R, Curran WJ Jr, Wu YL, et al. Lung cancer: current therapies and new targeted treatments. Lancet. 2017;389(10066):299–311. https://doi.org/10.1016/s0140-6736(16)30958-8.CrossRefPubMed

Lung cancer incidence and mortality with extended follow-up in the National Lung Screening Trial. J Thorac Oncol. 2019;14(10):1732–42. https://doi.org/10.1016/j.jtho.2019.05.044.

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424. https://doi.org/10.3322/caac.21492.CrossRef

Adjei AA. Lung cancer Worldwide. J Thorac Oncol. 2019;14(6):956. https://doi.org/10.1016/j.jtho.2019.04.001.CrossRefPubMed

Blandin Knight S, Crosbie PA, Balata H, Chudziak J, Hussell T, Dive C. Progress and prospects of early detection in lung cancer. Open Biol. 2017;7(9):170070. https://doi.org/10.1098/rsob.170070.CrossRefPubMedPubMedCentral

Rahal Z, El Nemr S, Sinjab A, Chami H, Tfayli A, Kadara H. Smoking and lung cancer: a geo-regional perspective. Front Oncol. 2017;7:194. https://doi.org/10.3389/fonc.2017.00194.CrossRefPubMedPubMedCentral

Pelosof L, Ahn C, Gao A, Horn L, Madrigales A, Cox J, et al. Proportion of never-smoker non-small cell lung cancer patients at three diverse institutions. J Natl Cancer Inst. 2017;109(7):djw295. https://doi.org/10.1093/jnci/djw295.CrossRefPubMedCentral

Gainor JF, Dardaei L, Yoda S, Friboulet L, Leshchiner I, Katayama R, et al. Molecular mechanisms of resistance to first- and second-generation ALK inhibitors in ALK-rearranged lung cancer. Cancer Discov. 2016;6(10):1118–33. https://doi.org/10.1158/2159-8290.Cd-16-0596.CrossRefPubMedPubMedCentral

Mayekar MK, Bivona TG. Current landscape of targeted therapy in lung cancer. Clin Pharmacol Ther. 2017;102(5):757–64. https://doi.org/10.1002/cpt.810.CrossRef

10.

Devarakonda S, Govindan R, Morgensztern D. Mastering the complex targeted therapy for non-small cell lung cancer. Cancer Cell. 2020. https://doi.org/10.1016/j.ccell.2020.07.011.CrossRefPubMedPubMedCentral

11.

Patop IL, Kadener S. circRNAs in cancer. Curr Opin Genet Dev. 2018;48:121–7. https://doi.org/10.1016/j.gde.2017.11.007.CrossRefPubMed

12.

Zhou R, Wu Y, Wang W, Su W, Liu Y, Wang Y, et al. Circular RNAs (circRNAs) in cancer. Cancer Lett. 2018;425:134–42. https://doi.org/10.1016/j.canlet.2018.03.035.CrossRefPubMed

13.

Lei B, Tian Z, Fan W, Ni B. Circular RNA: a novel biomarker and therapeutic target for human cancers. Int J Med Sci. 2019;16(2):292–301. https://doi.org/10.7150/ijms.28047.CrossRefPubMedPubMedCentral

14.

Li Z, Chen Z, Feng Y, Hu G, Jiang Y. CircMMP11 acts as a ce-circRNA in breast cancer progression by regulating miR-1204. Am J Transl Res. 2020;12(6):2585–99.PubMedPubMedCentral

15.

Wu Y, Wan X, Zhao X, Song Z, Xu Z, Tao Y, et al. MicroRNA-143 suppresses the proliferation and metastasis of human gastric cancer cells via modulation of STAT3 expression. Am J Transl Res. 2020;12(3):867–74.PubMedPubMedCentral

16.

Zhang HB, Sun LC, Ling L, Cong LH, Lian R. miR-143 suppresses the proliferation of NSCLC cells by inhibiting the epidermal growth factor receptor. Exp Ther Med. 2016;12(3):1795–802. https://doi.org/10.3892/etm.2016.3555.CrossRefPubMedPubMedCentral

17.

Tian S, Han G, Lu L, Meng X. Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis. World J Surg Oncol. 2020;18:56. https://doi.org/10.1186/s12957-020-01832-9.CrossRefPubMedPubMedCentral

18.

Xiang T, Jiang H, Zhang B, Liu G. CircFOXO3 functions as a molecular sponge for miR-143-3p to promote the progression of gastric carcinoma via upregulating USP44. Gene. 2020;753:144798. https://doi.org/10.1016/j.gene.2020.144798.CrossRefPubMed

Title: CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation
Authors: Juan Chen
Jiang Gong
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: NSCLC
NSCLC
Published in: Journal of Cardiothoracic Surgery / Issue 1/2021
Electronic ISSN: 1749-8090
DOI: https://doi.org/10.1186/s13019-021-01701-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

CircMMP11 overexpression predicts the poor survival of non-small cell lung cancer and downregulates miR-143 through methylation to suppress cell proliferation

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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