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01-09-2008 | Original Article

Novel transcript profiling of diffuse alveolar damage induced by hyperoxia exposure in mice: normalization by glyceraldehyde 3-phosphate dehydrogenase

Authors: Ichiroh Shimada, Kazuhiro Matsui, Bernd Brinkmann, Carsten Hohoff, Koichi Hiraga, Yoshiaki Tabuchi, Ichiro Takasaki, Ichiro Kato, Hiroshi Kawaguchi, Kumi Takasawa, Reiko Iida, Hisao Takizawa, Takasumi Matsuki

Published in: International Journal of Legal Medicine | Issue 5/2008

Abstract

Under mechanical ventilation with high-inspired oxygen concentration, diffuse alveolar damage was found to take place in some patients. To clarify the molecular pathophysiology of this condition, we investigated the time course of gene expression changes induced by hyperoxia exposure in mouse lung using real-time quantitative polymerase chain reaction (qPCR). Our results normalized by glyceraldehyde 3-phosphate dehydrogenase showed that mRNA levels of cysteine rich protein 61 (CYR61) and connective tissue growth factor (CTGF) were significantly upregulated, while those of surfactant-associated protein C (SFTPC), cytochrome P450, 2F2 (CYP2F2), Claudin 1, (CLDN1), membrane-associated zonula occludens protein-1 (ZO-1), lysozyme (LYZS), and P lysozyme structural (LZP-S) were significantly downregulated. Increasing level of mRNAs, each encoding CYR61 and CTGF, suggests a serious risk of fibrosing alveolitis. Decrease in levels of mRNAs for SFTPC, CYP2F2, CLDN1, ZO-1, LYZS, and LZP-S suggests alveolar dysfunction and disruption of the immune system. Moreover, we confirmed apoptotic conditions, such as significant upregulations of mRNA levels in Myc and Galectin-3. Hyperoxic condition probably yielded reactive oxygen species (ROS), which resulted in a malignant cycle of ROS production by Myc overexpression.

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Title: Novel transcript profiling of diffuse alveolar damage induced by hyperoxia exposure in mice: normalization by glyceraldehyde 3-phosphate dehydrogenase
Authors: Ichiroh Shimada
Kazuhiro Matsui
Bernd Brinkmann
Carsten Hohoff
Koichi Hiraga
Yoshiaki Tabuchi
Ichiro Takasaki
Ichiro Kato
Hiroshi Kawaguchi
Kumi Takasawa
Reiko Iida
Hisao Takizawa
Takasumi Matsuki
Publication date: 01-09-2008
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 5/2008
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-008-0226-6

At a glance: The STEP trials

Springer Medicine

Novel transcript profiling of diffuse alveolar damage induced by hyperoxia exposure in mice: normalization by glyceraldehyde 3-phosphate dehydrogenase

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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