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Acta Neuropathologica
Published in: Acta Neuropathologica 4/2010

01-04-2010 | Methods Paper

Novel myosin heavy chain immunohistochemical double staining developed for the routine diagnostic separation of I, IIA and IIX fibers

Authors: Olayinka Raheem, Sanna Huovinen, Tiina Suominen, Hannu Haapasalo, Bjarne Udd

Published in: Acta Neuropathologica | Issue 4/2010

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Abstract

The different histochemical ATPase properties of myosins separating the muscle fiber types have been utilized in diagnostic muscle biopsy routine for more than four decades. The ATPase staining method is rather laborious and has several disadvantages, such as weakening of staining over time and non-specific staining of capillaries, making the distinction of extremely atrophic muscle fibers difficult. We have developed a reliable and advanced immunohistochemical myosin double staining method for the identification of fiber types, including highly atrophic fibers in routine diagnostics. With this double staining method, we are able to distinguish among type I (ATPase type 1), IIA (ATPase type 2A), IIX (ATPase type 2B) and remodeled ATPase type 2C fibers expressing both fast and slow myosins using a one slide technique. Immunohistochemical double staining of myosin heavy chain isoforms can be used as an alternative for the conventional ATPase staining method in routine histopathology. The method provides even more detailed information of fast fiber subtypes and highly atrophic fibers on one single slide.
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Metadata
Title
Novel myosin heavy chain immunohistochemical double staining developed for the routine diagnostic separation of I, IIA and IIX fibers
Authors
Olayinka Raheem
Sanna Huovinen
Tiina Suominen
Hannu Haapasalo
Bjarne Udd
Publication date
01-04-2010
Publisher
Springer-Verlag
Published in
Acta Neuropathologica / Issue 4/2010
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI
https://doi.org/10.1007/s00401-010-0643-8

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