Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
European Radiology
Published in: European Radiology 9/2010

01-09-2010 | Molecular Imaging

Non-invasive tracking of human haemopoietic CD34+ stem cells in vivo in immunodeficient mice by using magnetic resonance imaging

Authors: Markus Niemeyer, Robert A. J. Oostendorp, Markus Kremer, Sandra Hippauf, Volker R. Jacobs, Hansjörg Baurecht, Georg Ludwig, Guido Piontek, Viktoria Bekker-Ruz, Sebastian Timmer, Ernst J. Rummeny, Marion Kiechle, Ambros J. Beer

Published in: European Radiology | Issue 9/2010

Login to get access

Abstract

Objective

To assess migration of CD34+ human stem cells to the bone marrow of athymic mice by using magnetic resonance (MR) imaging and Resovist, a contrast agent containing superparamagnetic iron oxide (SPIO) particles.

Methods

All animal and human procedures were approved by our institution’s ethics committee, and women had given consent to donate umbilical cord blood (UCB). Balb/c-AnN Foxn1nu/Crl mice received intravenous injection of 1 × 106 (n = 3), 5 × 106 (n = 3) or 1 × 107 (n = 3) human Resovist-labelled CD34+ cells; control mice received Resovist (n = 3). MR imaging was performed before, 2 and 24 h after transplantation. Signal intensities of liver, muscle and bone marrow were measured and analysed by ANOVA and post hoc Student’s t tests. MR imaging data were verified by histological and immunological detection of both human cell surface markers and carboxydextran-coating of the contrast agent.

Results

CD34+ cells were efficiently labelled by Resovist without impairment of functionality. Twenty-four hours after administration of labelled cells, MR imaging revealed a significant signal decline in the bone marrow, and histological and immunological analyses confirmed the presence of transplanted human CD34+ cells.

Conclusion

Intravenously administered Resovist-labelled CD34+ cells home to bone marrow of mice. Homing can be tracked in vivo by using clinical 1.5-T MR imaging technology.
Literature
1.
Kondo M, Wagers AJ, Manz MG et al (2003) Biology of hematopoietic stem cells and progenitors: implications for clinical application. Annu Rev Immunol 21:759–806CrossRefPubMed
2.
Reya T, Morrison SJ, Clarke MF et al (2001) Stem cells, cancer, and cancer stem cells. Nature 414:105–111CrossRefPubMed
3.
Bradley MB, Cairo MS (2005) Cord blood immunology and stem cell transplantation. Hum Immunol 66:431–446CrossRefPubMed
4.
Eapen M, Rubinstein P, Zhang MJ et al (2007) Outcomes of transplantation of unrelated donor umbilical cord blood and bone marrow in children with acute leukaemia: a comparison study. Lancet 369:1947–1954CrossRefPubMed
5.
Civin CI, Trischmann T, Kadan NS et al (1996) Highly purified CD34-positive cells reconstitute hematopoiesis. J Clin Oncol 14:2224–2233PubMed
6.
Wagner JE, Barker JN, DeFor TE et al (2002) Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and nonmalignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival. Blood 100:1611–1618PubMed
7.
Elmaagacli AH, Peceny R, Steckel N et al (2003) Outcome of transplantation of highly purified peripheral blood CD34+ cells with T-cell add-back compared with unmanipulated bone marrow or peripheral blood stem cells from HLA-identical sibling donors in patients with first chronic phase chronic myeloid leukemia. Blood 101:446–453CrossRefPubMed
8.
Lanzkron SM, Collector MI, Sharkis SJ (1999) Hematopoietic stem cell tracking in vivo: a comparison of short-term and long-term repopulating cells. Blood 93:1916–1921PubMed
9.
10.
Barkhausen J, Quick HH, Lauenstein T et al (2001) Whole-body MR imaging in 30 seconds with real-time true FISP and a continuously rolling table platform: feasibility study. Radiology 220:252–256PubMed
11.
Choi S, Tang XW, Cory DG (1997) Constant time imaging approaches to NMR microscopy. Int J Imaging Syst Technol 8:263–276CrossRef
12.
Daldrup-Link HE, Rudelius M, Oostendorp RA et al (2003) Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology 228:760–767CrossRefPubMed
13.
Rudelius M, drup-Link HE, Heinzmann U et al (2003) Highly efficient paramagnetic labelling of embryonic and neuronal stem cells. Eur J Nucl Med Mol Imaging 30:1038–1044CrossRefPubMed
14.
Arbab AS, Yocum GT, Wilson LB et al (2004) Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability. Mol Imaging 3:24–32CrossRefPubMed
15.
Frank JA, Miller BR, Arbab AS et al (2003) Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents. Radiology 228:480–487CrossRefPubMed
16.
Frank JA, Anderson SA, Kalsih H et al (2004) Methods for magnetically labeling stem and other cells for detection by in vivo magnetic resonance imaging. Cytotherapy 6:621–625CrossRefPubMed
17.
Daldrup-Link HE, Rudelius M, Piontek G et al (2005) Migration of iron oxide-labeled human hematopoietic progenitor cells in a mouse model: in vivo monitoring with 1.5-T MR imaging equipment. Radiology 234:197–205CrossRefPubMed
18.
Maxwell DJ, Bonde J, Hess DA et al (2008) Fluorophore-conjugated iron oxide nanoparticle labeling and analysis of engrafting human hematopoietic stem cells. Stem Cells 26:517–524CrossRefPubMed
19.
Ahmed F, Ings SJ, Pizzey AR et al (2004) Impaired bone marrow homing of cytokine-activated CD34+ cells in the NOD/SCID model. Blood 103:2079–2087CrossRefPubMed
20.
Oostendorp RA, Gilfillan S, Parmar A et al (2008) Oncostatin M-mediated regulation of KIT-ligand-induced extracellular signal-regulated kinase signaling maintains hematopoietic repopulating activity of Lin-CD34+CD133+ cord blood cells. Stem Cells 26:2164–2172CrossRefPubMed
21.
Breems DA, Blokland EA, Neben S et al (1994) Frequency analysis of human primitive haematopoietic stem cell subsets using a cobblestone area forming cell assay. Leukemia 8:1095–1104PubMed
22.
O’Brien RG, Muller KE (1993) Unified power analysis for t-tests through multivariate hypotheses. In: Applied analysis of variance in behavioral science. Marcel Dekker, New York, pp 297–344
23.
Bonde J, Hess DA, Nolta JA (2004) Recent advances in hematopoietic stem cell biology. Curr Opin Hematol 11:392–398CrossRefPubMed
24.
Hacein-Bey-Abina S, Von KC, Schmidt M et al (2003) A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. N Engl J Med 348:255–256CrossRefPubMed
25.
26.
Arbab AS, Yocum GT, Kalish H et al (2004) Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood 104:1217–1223CrossRefPubMed
27.
Arbab AS, Janic B, Knight RA et al (2008) Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings. FASEB J 22:3234–3246CrossRefPubMed
Metadata
Title
Non-invasive tracking of human haemopoietic CD34+ stem cells in vivo in immunodeficient mice by using magnetic resonance imaging
Authors
Markus Niemeyer
Robert A. J. Oostendorp
Markus Kremer
Sandra Hippauf
Volker R. Jacobs
Hansjörg Baurecht
Georg Ludwig
Guido Piontek
Viktoria Bekker-Ruz
Sebastian Timmer
Ernst J. Rummeny
Marion Kiechle
Ambros J. Beer
Publication date
01-09-2010
Publisher
Springer-Verlag
Published in
European Radiology / Issue 9/2010
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI
https://doi.org/10.1007/s00330-010-1773-z

Other articles of this Issue 9/2010

European Radiology 9/2010 Go to the issue

Computer Applications

APPC—A new standardised coding system for trans-organisational PACS retrieval

Interventional

Small nodular hepatocellular carcinoma treated by laser thermal ablation in high risk locations: preliminary results

Hepatobiliary-Pancreas

Hepatic focal nodular hyperplasia: contrast-enhanced ultrasound findings with emphasis on lesion size, depth and liver echogenicity

Breast

Design-related bias in estimates of accuracy when comparing imaging tests: examples from breast imaging research

Letter to the Editor

Letter to the Editor regarding: Comparison of dual-source CT angiography and MR angiography in preoperative evaluation of intra- and extracranial vessels: a pilot study

Hepatobiliary-Pancreas

Liver virtual non-enhanced CT with dual-source, dual-energy CT: a preliminary study