Top

Published in:

01-08-2004 | Original Article

Nitrogen laser irradiation (337 nm) causes temporary inactivation of clinical isolates of Mycobacterium tuberculosis

Authors: Alok Dube, K. Jayasankar, L. Prabakaran, V. Kumar, P. K. Gupta

Published in: Lasers in Medical Science | Issue 1/2004

Abstract

We have investigated the effect of nitrogen laser irradiation (337 nm) on viability of clinical isolates of Mycobacterium tuberculosis. Bacteria were exposed to a nitrogen laser (average power 2.0 mW) in vitro at power density of 70 ± 0.7 W/m² for 0–30 min, and the cell viability was determined by luciferase reporter phage (LRP) assay. Immediately after laser exposure, all the clinical isolates investigated showed a dose-dependent decrease in cell viability. However, when the laser-exposed isolates were incubated in broth medium for 3 days, most of these showed significant recovery from laser-induced damage. Addition of 5.0 μg/ml acriflavine (a DNA repair inhibitor) in the incubation medium had no significant effect on recovery. This suggests that DNA damage may not be involved in the cell inactivation. Electron paramagnetic resonance (EPR) studies using 5-doxyl strearic acid (5-DS) as a probe suggest alterations in lipid regions of the cell wall. Implications of these results for understanding therapeutic effect of nitrogen laser on drug-resistant tuberculosis are discussed.

Finsen NR (1901) The chemical rays of light and smallpox. In: Finsen NR (ed) Phototherapy. Arnold, New York (J. H. Sequiera, translator)

Dreher W, Domanska B (1968) Bactericidal effect of ultraviolet rays of the quartz lamp on tuberculi bacilli H37Rv and on acid-fast bacilli cultured from sputum of a patient. Gruzlica 36:181–183PubMed

Eshankhanov M, Khodzhaeva MI, Takhirov ON , Priluk KP (1989) Nitrogen laser in the therapy of lung destructive tuberculosis. In: Proceeding of the international conference, Tashkent, part 3, p 206

Puri MM, Myneed VP, Jain RC (1995) Nitrogen and helium-neon laser therapy in the treatment of drug-resistant pulmonary tuberculosis. Laser Ther 7:123–128

Bhagawanani NS, Bhatia GC, Sharma N (1996) Low level nitrogen laser therapy in pulmonary tuberculosis. J Clin Laser Med Surg 14:23–25PubMed

Puri MM, Singal R, Jaisawal A, Gupta K, Jain RC (1997) Case reports on the role of laser therapy in the treatment of tuberculosis of the lymph nodes. Laser Ther 9:55–58

Singh HMP, Bajpai A, Bisarya BN, Bhargava KD (1997) Low level laser therapy (LLLT) with nitrogen laser and helium-neon laser in multiple drug resistant pulmonary tuberculosis: a preliminary study. Laser Ther 9:173–180

Sachdeva R, Bhagwanani NS, Chitnis DS (1995) The nitrogen laser inhibits the growth of wide range of microbes in vitro. Laser Ther 7:23–26

Sachdeva R, Bhagwanani NS, Chitnis DS (1995) Low incident energy levels enhances the biocidal activity of human neutrophils on internalized bacteria: an in vitro study. Laser Ther 7:107–112

10.

Vlassov VV, Pechatnikov LM, MacLehose HG (2003) Low level laser therapy for treating tuberculosis (Cochrane review). In: The Cochrane library, Issue 2, Oxford

11.

Billard P, DuBow MS (1998) Bioluminescence-based assays for detection and characterization of bacteria and chemicals in clinical laboratories. Clin Biochem 31:1–14CrossRefPubMed

12.

Riska PF, Su Y, Bardarov S, Freundlich L, Sarkis G, Hatfull GF, Carriere G, Kumar V, Chan J, Jacobs WR Jr (1999) Rapid film-based determination of antibiotic susceptibilities of Mycobacterium tuberculosis by using a Luciferase reporter phage and the Bronx Box. J Clin Microbiol 37:1144–1149PubMed

13.

Liu J, Rosenberg EY, Nikaido H (1995) Fluidity of the lipid domain of cell wall from Mycobacterium chelonae. Proc Natl Acad Sci U S A 92:11254–11258PubMed

14.

Favre A, Hajnsdorf E, Thiam K, Caldeira de Araujo A (1985) Mutagenesis and growth delay induced in Escherichia coli by near ultraviolet radiations. Biochimie 67:335–342PubMed

15.

Kramer GF, Ames BN (1987) Oxidative mechanism of toxicity of low intensity near-UV light in Salmonella typhimurium. J Bacteriol 169:2259–2266PubMed

16.

Taber H, Pomerantz BJ, Halfenger GM (1978) Near ultraviolet induction of growth delay studied in a menaquinone-deficient mutant of Bacillus subtilis. Photochem Photobiol 28:191–196PubMed

17.

Oppezzo OJ, Pizarro RA (2001) Sublethal effects of ultraviolet A radiation on Enterobacter cloacae. J Photochem Photobiol B 62:158–165CrossRefPubMed

18.

Webb RB, Brown MS (1976) Sensitivity of Escherichia coli differing in repair capability to Far UV, Near UV and visible radiations. Photochem Photobiol 24:425–432PubMed

19.

Singh VP, Shanker S (1994) Irradiation effect of acriflavin on UV inactivation of Rhizobium phaseoli. Microbios 78:185–197

20.

Bhattacharyya S, Paul AK (2001) Enhanced UV sensitivity of Thiobacillus ferrooxidans resulting from caffeine and acriflavine treatment of irradiated cells. Curr Microbiol 43:149–153CrossRefPubMed

21.

Fortini P, Pascucci B, Parlanti E, D’Errico M, Simonelli V, Dogliotti E (2003) 8-Oxoguanine DNA damage: at the crossroad of alternative repair pathways. Mutat Res 531:127–139CrossRefPubMed

22.

Michaelis M, Langer K, Vogel JU, Kreuter J, Rabenau H, Doerr HW, Cinatl J (2002) In vitro antiviral activity of aphidicolin and its derivates. Synergistic effects of aphidicolin with other antiviral drugs. Arzneimittelforschung 52:393–399PubMed

23.

Shiraki K, Rapp F (1988) Effects of caffeine on herpes simplex virus. Interviol 29:235–240

24.

Fernandez RO, Pizarro RA (1996) Lethal effect induced in Pseudomonas aeruginosa exposed to ultraviolet-A radiation. Photochem Photobiol 64:334–339PubMed

25.

Brennan PJ (2003) Structure, function and biogenesis of the cell wall of Mycobacterium tuberculosis. Tuberculosis 83:91–97CrossRefPubMed

26.

Mody R, Mody B, Dave P (1991) Damage to the plasma membrane in Escherichia coli K-12 induced by far-ultraviolet radiation and its repair. Radiat Res 127:156–163PubMed

27.

Pizarro RA, Orce LV (1988) Membrane damage and recovery associated with growth delay induced by near-UV radiation in Escherichia coli K-12. Photochem Photobiol 47:391–397PubMed

28.

Chamberlain J, Moss SH (1987) Lipid peroxidation and other membrane damage produced in Escherichia coli K1060 by near-UV radiation and deuterium oxide. Photochem Photobiol 45:625–630PubMed

29.

Muela A, Garcia-Bringas JM, Seco C, Arana I, Barcina I (2002) Participation of oxygen and role of exogenous and endogenous sensitizers in the photoinactivation of Escherichia coli by photosynthetically active radiation, UV-A and UV-B. Microb Ecol 44:354–364CrossRefPubMed

30.

Deretic V, Philipp W, Dhandayuthapani S, Mudd MH, Curcic R, Garbe T, Heym B, Via LE, Cole ST (1995) Mycobacterium tuberculosis is a natural mutant with an inactivated oxidative-stress regulatory gene: implications for sensitivity to isoniazid. Mol Microbiol 17:889–900CrossRefPubMed

31.

Master SS, Springer B, Sander P, Boettger EC, Deretic V, Timmins GS (2002) Oxidative stress response genes in Mycobacterium tuberculosis: role of ahpC in resistance to peroxynitrite and stage-specific survival in macrophages. Microbiol 148:3139–3144

32.

Jaeger T, Budde H, Floh L, Menge U, Singh M, Trujillo M, Radi R (2004) Multiple thioredoxin-mediated routes to detoxify hydroperoxides in Mycobacterium tuberculosis. Arch Biochem Biophys 423:182–191CrossRefPubMed

Title: Nitrogen laser irradiation (337 nm) causes temporary inactivation of clinical isolates of Mycobacterium tuberculosis
Authors: Alok Dube
K. Jayasankar
L. Prabakaran
V. Kumar
P. K. Gupta
Publication date: 01-08-2004
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 1/2004
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-004-0304-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Nitrogen laser irradiation (337 nm) causes temporary inactivation of clinical isolates of Mycobacterium tuberculosis

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2004

The 7th Annual Lasercare Educational Day

Surgical treatment of ranula with carbon dioxide laser radiation

Side-viewing fiberoptic catheter for biospectroscopy applications

The 980-nm diode laser for brain surgery: histopathology and recovery period

Low-power laser biostimulation enhances nerve repair after end-to-side neurorrhaphy: a double-blind randomized study in the rat median nerve model

Laser Florence 2004