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World Journal of Surgery
Published in: World Journal of Surgery 4/2007

01-04-2007

Nitric Oxide Accelerates the Recovery from Burn Wounds

Authors: Haifeng Zhu, PhD, Bian Ka, MD, PhD, Ferid Murad, MD, PhD

Published in: World Journal of Surgery | Issue 4/2007

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Abstract

A full-thickness burn wound model was used to evaluate the effects of a topically applied gel-based nitric oxide donor on wound healing in rats. The histological study demonstrated that the nitric oxide (NO) application significantly promoted re-epithelization that resulted in a fast recovery of burn wound. The histological sections further revealed that inflammatory cell infiltration in the NO-treated group was significantly increased in comparison to the control group. The enhanced accumulation of inflammatory cells resulted in a higher expression of myeloperoxidase (MPO) that was detected with imunoblotting. An immunohistochemistry study with CD31, a specific marker for endothelial cells, indicated that NO treatment markedly stimulated angiogenesis. Evaluation of collagen synthesis by immunohistochemistry with procollagen antibody demonstrated a significantly increased collagen synthesis in NO-treated wound bed. We concluded that NO treatment promoted re-epithelialization and wound closure by means of enhanced inflammatory cell infiltration, and that it promoted angiogenesis and facilitated collagen synthesis in the wound bed.
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Metadata
Title
Nitric Oxide Accelerates the Recovery from Burn Wounds
Authors
Haifeng Zhu, PhD
Bian Ka, MD, PhD
Ferid Murad, MD, PhD
Publication date
01-04-2007
Publisher
Springer-Verlag
Published in
World Journal of Surgery / Issue 4/2007
Print ISSN: 0364-2313
Electronic ISSN: 1432-2323
DOI
https://doi.org/10.1007/s00268-007-0727-3

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