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Pediatric Surgery International

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01-09-2010 | Original Article

NF-κB related abnormal hyper-expression of iNOS and NO correlates with the inflammation procedure in biliary atresia livers

Authors: Lei Huang, Xin-Min Si, Jie-Xiong Feng

Published in: Pediatric Surgery International | Issue 9/2010

Abstract

Objective

To study the expression of inducible nitric oxide synthase (iNOS) and its related regulators in biliary atresia (BA) livers and exlpore their relationships with the inflammation pathway in BA livers.

Method

The iNOS expression in livers of 38 cases of BA children, 15 cases of neonatal cholestasis (NC) children, and 18 cases of normal control were, respectively, examined and total nitric oxide (NO) metabolites concentration of all samples were calculated by a colorimetric method based on Griess reaction. The TdT-mediated dupt biotin nick end labeling (TUNEL) method was used to label the apoptotic bile duct epithelial cells and hepatocytes. The western blotting and immunohistochemistry methods to semi-quantitatively analyze the nuclear factor-κB (NF-κB) expression of each group were also used.

Results

The iNOS expression intensity and total NO metabolites concentration of BA group (0.30 ± 0.08, 90.40 ± 12.46 μmol/L) were significantly higher than those of NC and normal control groups, P < 0.01. Correlation analysis showed a strong positive correlation between the serum AST level (152.76 ± 29.59 U/L) and total NO metabolites concentration in BA group. Compared with the NC (32.47 ± 5.55) and normal control (20.72 ± 5.63) groups, a significantly higher apoptosis rate of intrahepatic bile duct epithelial cells was found in BA group (54.00 ± 11.67) that tightly correlated with the iNOS intensity (r = 0.99, P < 0.01). The NF-κB intensity of BA group was also significantly higher than that of NC and normal control groups and had a strong positive correlation with the iNOS intensity (r = 0.97, P < 0.01).

Conclusion

The abnormal hyper-expression of iNOS may play an important role in mediating the inflammation procedure in BA livers. This change perhaps has some relationship with the high expression of NF-κB and NO. These regulators can up-regulate the apoptosis of bile duct epithelial cells in BA livers and cause the damage to liver tissues.

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Title: NF-κB related abnormal hyper-expression of iNOS and NO correlates with the inflammation procedure in biliary atresia livers
Authors: Lei Huang
Xin-Min Si
Jie-Xiong Feng
Publication date: 01-09-2010
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 9/2010
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-010-2683-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

NF-κB related abnormal hyper-expression of iNOS and NO correlates with the inflammation procedure in biliary atresia livers

Abstract

Objective

Method

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Method

Results

Conclusion

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