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01-09-2016 | Original Paper

Neuroprotective Effects of Germinated Brown Rice in Rotenone-Induced Parkinson’s-Like Disease Rats

Authors: Supin Chompoopong, Sunit Jarungjitaree, Tideeporn Punbanlaem, Thanaporn Rungruang, Sukumal Chongthammakun, Aikkarach Kettawan, Thongchai Taechowisan

Published in: NeuroMolecular Medicine | Issue 3/2016

Abstract

The effects of germinated brown rice (GBR) on the motor deficits and the dopaminergic (DA) cell death were investigated in Parkinson’s-like disease (PD) rats. Reactive oxidative species generated by chronic subcutaneous injection of rotenone (RT) lead to neuronal apoptosis particularly in the nigrostriatal DA system and produce many features of PD, bradykinesis, postural instability and rigidity. In this study, 4-phenylbutyric acid (4-PBA), previously reported to inhibit RT-induced DA cell death, was used as the positive control. Results show that pretreatment with GBR as well as 4-PBA significantly enhanced the motor activity after RT injection, and GBR affected significantly in open field test, only in the ambulation but not the mobility duration, and ameliorated the time to orient down (t-turn) and total time to descend the pole (t-total) in pole test as compared to RT group, but significantly lowered both t-turn and t-total only in 4-PBA group. The percentage of apoptotic cells in brain measured by flow cytometry and the inflammatory effect measured by ELISA of TNF-α showed significant increase in RT group as compared to the control (CT) group at P < 0.05. Apoptotic cells in RT group (85.98 %) showed a significant (P < 0.05) increase versus CT group (17.50 %), and this effect was attenuated in GBR+RT group by decreasing apoptotic cells (79.32 %), whereas, increased viable cells (17.94 %) versus RT group (10.79 %). GBR in GBR + RT group could decrease TNF-α both in the serum and in brain. In summary, GBR showed a neuroprotective effect in RT-induced PD rats, and it may be useful as a value-added functional food to prevent neurodegenerative disease or PD.

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Title: Neuroprotective Effects of Germinated Brown Rice in Rotenone-Induced Parkinson’s-Like Disease Rats
Authors: Supin Chompoopong
Sunit Jarungjitaree
Tideeporn Punbanlaem
Thanaporn Rungruang
Sukumal Chongthammakun
Aikkarach Kettawan
Thongchai Taechowisan
Publication date: 01-09-2016
Publisher: Springer US
Published in: NeuroMolecular Medicine / Issue 3/2016
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-016-8427-5

At a glance: The STEP trials

Springer Medicine

Neuroprotective Effects of Germinated Brown Rice in Rotenone-Induced Parkinson’s-Like Disease Rats

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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