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01-12-2023 | Neuroblastoma | Original Article

Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells

Authors: Ayano Hidaka, Shota Uekusa, Takashi Hosokawa, Hide Kaneda, Tomohiko Kazama, Kazuhiro Hagikura, Shuichiro Uehara, Tsugumichi Koshinaga, Taro Matsumoto

Published in: Pediatric Surgery International | Issue 1/2023

Abstract

Purpose

Mesenchymal stem cells (MSCs) can induce differentiation of neuroblastoma (NB) cells. Properties of dedifferentiated fat cells (DFATs) are similar to those of MSCs. Here, we investigated whether DFATs can induce NB cell differentiation and suppress cell proliferation.

Methods

DFATs were obtained from mature adipocytes isolated from adipose tissue from a ceiling culture. NB cells were cultured in a medium with or without DFATs and, subsequently, cultured in a DFAT-conditioned medium (CM) with or without phosphatidylinositol 3-kinase (PI3K) inhibitor. The neurite lengths were measured, and mRNA expression levels of the neurofilament (NF) and tubulin beta III (TUBβ3) were assessed using quantitative real-time RT-PCR. Cell viability was assessed using the WST-1 assay.

Results

NB cells cultured with DFATs caused elongation of the neurites and upregulated the expression of NF and Tubβ3. NB cells cultured in DFAT-CM demonstrated increased cell viability. NB cells cultured with DFAT-CM and PI3K inhibitors suppressed cell viability. NB cells cultured with DFAT-CM and PI3K inhibitor demonstrated increased neurite length and expression, and upregulation of Tubβ3.

Conclusion

The combined use of DFAT-CM and PI3K inhibitors suppresses the proliferation of NB cells and induces their differentiation. Thus, DFAT may offer new insights into therapeutic approaches in neuroblastoma.

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Title: Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells
Authors: Ayano Hidaka
Shota Uekusa
Takashi Hosokawa
Hide Kaneda
Tomohiko Kazama
Kazuhiro Hagikura
Shuichiro Uehara
Tsugumichi Koshinaga
Taro Matsumoto
Publication date: 01-12-2023
Publisher: Springer Berlin Heidelberg
Keywords: Neuroblastoma
Neuroblastoma
Published in: Pediatric Surgery International / Issue 1/2023
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-022-05304-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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