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01-12-2013 | Letter

Need to evaluate the performance of real-time PCR assays for the quantitation of cytomegalovirus DNA load in lower respiratory tract specimens

Authors: María Ángeles Clari, Estela Giménez, Gerardo Aguilar, Isabel Corrales, Juan Alberola, David Navarro

Published in: Critical Care | Issue 6/2013

Excerpt

There is an increasing appreciation for the potential clinical value of the quantitation of cytomegalovirus (CMV) DNA in the lower respiratory tract in critically ill patients lacking canonical immunosuppression, in view of the possible pathogenic role of CMV in these patients [1]. No data have been published on the analytical performance of real-time PCR assays for this purpose. …

Kalil AC, Florescu DF: Prevalence and mortality associated with cytomegalovirus infection in nonimmunosuppressed patients in the intensive care unit. Crit Care Med 2009, 37: 2350-2358. 10.1097/CCM.0b013e3181a3aa43CrossRefPubMed

Hirsch HH, Lautenschlager I, Pinsky BA, Cardeñoso L, Aslam S, Cobb B, Vilchez RA, Valsamakis A: An international multicenter performance analysis of CMV viral load tests. Clin Infect Dis 2013, 56: 367-373. 10.1093/cid/cis900PubMedCentralCrossRefPubMed

Chilet M, Aguilar G, Benet I, Belda J, Tormo N, Carbonell JA, Clari MA, Costa E, Navarro D: Virological and immunological features of active cytomegalovirus infection in nonimmunosuppressed patients in a surgical and trauma intensive care unit. J Med Virol 2010, 82: 1384-1391. 10.1002/jmv.21825CrossRefPubMed

Fryer JF, Heath AB, Anderson R, Minor PD: Collaborative Study Group: Collaborative Study to Evaluate the Proposed 1^stWHO International Standard for Human Cytomegalovirus (HCMV) for Nucleic Acid Amplification (NAT)-based Assays . In WHO ECBS Report 2010, WHO/BS/10.2138. Geneva: WHO Press; 2010.

COBAS® TaqMan® CMV Test FDA Approved Package Insert. Roche Diagnostics: Mannheim; 2012.

Title: Need to evaluate the performance of real-time PCR assays for the quantitation of cytomegalovirus DNA load in lower respiratory tract specimens
Authors: María Ángeles Clari
Estela Giménez
Gerardo Aguilar
Isabel Corrales
Juan Alberola
David Navarro
Publication date: 01-12-2013
Publisher: BioMed Central
Published in: Critical Care / Issue 6/2013
Electronic ISSN: 1364-8535
DOI: https://doi.org/10.1186/cc13119

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Need to evaluate the performance of real-time PCR assays for the quantitation of cytomegalovirus DNA load in lower respiratory tract specimens

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Keynote webinar | Spotlight on medication adherence

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