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BMC Complementary Medicine and Therapies

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Open Access 01-12-2021 | Naloxone | Research article

Evaluation of antinociceptive activity of Ilex dipyrena Wall. in mice

Authors: Amjad Ali, Abdul Nasir, Syed Wadood Ali Shah, Atif Ali Khan Khalil, Mi-jeong Ahn, Syed Muhammad Mukarram Shah, Fazli Subhan, Muhammad Faheem, Wasim Sajjad, Mohammad Shoaib, Saeed Ahmad, Nausheen Nazir, Mohammad Nisar

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

In order to find a new natural resource for pain-relief, the analgesic effects of Ilex dipyrena crude extract, fractions, and subfractions were evaluated in in-vivo mouse models with possible mechanism of action.

Methods

Analgesic effects of crude extract (100 and 200 mg/kg body weight), fractions and subfractions (75 mg/kg body weight) were screened using heat-induced (tail-immersion and hot plate test) and chemical-induced (formalin and acetic acid) nociception models in mice. The samples were also tested for the elucidation of a possible mechanism through opioidergic and GABAergic systems.

Results

The administration of crude extract, fractions and subfractions produced analgesic responses in acetic acid, formalin, tail immersion, and hot plate model for pain similar to those obtained with the standard. Naloxone antagonized the antinociceptive effects of the tested samples, whereas bicuculline showed partial inhibition. Considering the analgesic response, crude extract, fractions, and subfractions demonstrated promising inhibitory activity against all test models for pain, which was further supported by the possible involvement of opioidergic and GABAergic systems.

Conclusion

The results suggest that this plant may be useful in the development of new analgesic drugs. Further research with regard to the isolation of bioactive compounds is required to verify these findings.

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Title: Evaluation of antinociceptive activity of Ilex dipyrena Wall. in mice
Authors: Amjad Ali
Abdul Nasir
Syed Wadood Ali Shah
Atif Ali Khan Khalil
Mi-jeong Ahn
Syed Muhammad Mukarram Shah
Fazli Subhan
Muhammad Faheem
Wasim Sajjad
Mohammad Shoaib
Saeed Ahmad
Nausheen Nazir
Mohammad Nisar
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Naloxone
Tramadol
Analgesics in Dentistry
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03357-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluation of antinociceptive activity of Ilex dipyrena Wall. in mice

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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