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01-03-2012 | Original Paper

Myelin specific cells infiltrate MCAO lesions and exacerbate stroke severity

Authors: Xuefang Ren, Kozaburo Akiyoshi, Marjorie R. Grafe, Arthur A. Vandenbark, Patricia D. Hurn, Paco S. Herson, Halina Offner

Published in: Metabolic Brain Disease | Issue 1/2012

Abstract

Although inflammatory responses increase stroke severity, the role of immune cells specific for central nervous system (CNS) antigens remains controversial. Disruption of the blood–brain barrier (BBB) during stroke allows CNS antigens to leak into the peripheral circulation and enhances access of circulating leukocytes to the brain, including those specific for CNS antigens such as myelin oligodendrocyte glycoprotein (MOG) that can induce experimental autoimmune encephalomyelitis (EAE). We here demonstrate for the first time that myelin reactive splenocytes specific for MOG transferred into severe combined immunodeficient (SCID) mice can migrate into the infarct hemisphere of recipients subjected to 60 min middle cerebral artery occlusion (MCAO) and 96 h reperfusion; moreover these cells exacerbate infarct volume and worsen neurological deficits compared to animals transferred with naïve splenocytes. These findings indicate that autoimmunity in the CNS can exert detrimental injury on brain cells and worsen the damage from ischemic stroke.

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Title: Myelin specific cells infiltrate MCAO lesions and exacerbate stroke severity
Authors: Xuefang Ren
Kozaburo Akiyoshi
Marjorie R. Grafe
Arthur A. Vandenbark
Patricia D. Hurn
Paco S. Herson
Halina Offner
Publication date: 01-03-2012
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 1/2012
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-011-9267-5

At a glance: The STEP trials

Springer Medicine

Myelin specific cells infiltrate MCAO lesions and exacerbate stroke severity

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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