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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2015 | Research

MTA1 promotes metastasis of MPM via suppression of E-cadherin

Authors: Caihua Xu, Fei Hua, Yihuan Chen, Haoyue Huang, Wenxue Ye, Yunsheng Yu, Zhenya Shen

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2015

Abstract

Background

Metastasis-associated gene 1(MTA1) has been identified as an oncogene in many tumors, and aberrant MTA1 expression has been linked to carcinogenesis and metastasis. We aim to investigate the mechanism of MTA1 and metastasis in malignant pleural mesothelioma (MPM).

Methods

Real-time polymerase chain reaction (PCR) and immunohistochemical staining were employed to detect MTA1 and E-cadherin expression in MPM tissues and corresponding adjacent tissues. Stable clone with knock-down of MTA1 was generated with shRNA via lentivirus technology in MPM cell lines. Wound-healing assay, transwell assay and PCR array were carried out for detecting invasion and migration of MPM cells. Luciferase reporter assay was performed to validate the effect of MTA1 on E-cadherin.

Results

MTA1 expression is up-regulated in MPM and shown a negative correlation with E-cadherin expression. MTA1 could enhance the invasion and migration of MPM cells via suppressing the expression of E-cadherin. MTA1 overexpression is associated with pathology, metastasis and survival rate of MPM patients.

Conclusions

MTA1 plays an important role in Epithelial-to-mesenchymal transition (EMT) to promote metastasis via suppressing E-cadherin expression, resulting in a poor prognosis in MPM. MTA1 is a novel biomarker and indicative of a poor prognosis in MPM patients.

Available only for authorised users

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Title: MTA1 promotes metastasis of MPM via suppression of E-cadherin
Authors: Caihua Xu
Fei Hua
Yihuan Chen
Haoyue Huang
Wenxue Ye
Yunsheng Yu
Zhenya Shen
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2015
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/s13046-015-0269-8

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Abstract

Background

Methods

Results

Conclusions

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