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01-05-2011 | Molecular Imaging

MR Imaging of activated hepatic stellate cells in liver injured by CCl₄ of rats with integrin-targeted ultrasmall superparamagnetic iron oxide

Authors: Qing-Bing Wang, Yu Han, Ting-Ting Jiang, Wei-Min Chai, Ke-Min Chen, Bing-Ya Liu, Li-Fu Wang, Chunfu Zhang, Deng-Bin Wang

Published in: European Radiology | Issue 5/2011

Abstract

Objective

To demonstrate the feasibility of the ultrasmall superparamagnetic iron oxide (USPIO) modified by cyclo (Arg-Gly-Asp-Try-Cys) peptide (c(RGDyC)-USPIO) for targeting hepatic stellate cells (HSCs).

Materials and Methods

A c(RGDyC)-USPIO probe was prepared by conjugating c(RGDyC) with USPIO through a thiol-maleinide interaction. The specificity of c(RGDyC)-USPIO for HSCs was investigated in vitro. In vivo, normal and fibrosis rats were treated with either c(RGDyC)-USPIO or USPIO, and magnetic resonance imaging (MRI) of the rats performed after administration of the probes for 4 h. The T2 relaxation times changes before and after probe injection were analyzed and the locations of probes in normal or injured mice were identified histologically.

Results

The hydrodynamic size of c(RGDyC)-USPIO was 13 ± 3 nm. HSCs took up more specific probes than plain ones. The reduction of T2 relaxation times in fibrosis rat by c(RGDyC)-USPIO was much greater than that by USPIO (P < 0.05). Prussian blue staining and transmission electron microscopy of the injured rat liver treated with c(RGDyC) demonstrated that c(RGDyC)-USPIO were specifically engulfed by the activated HSCs.

Conclusion

In vivo cellular targeted imaging of activated HSCs in liver fibrosis using c(RGDyC)-USPIO targeting α_vβ₃ integrins was feasible using a clinical 1.5-Tesla MR system.

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Title: MR Imaging of activated hepatic stellate cells in liver injured by CCl4 of rats with integrin-targeted ultrasmall superparamagnetic iron oxide
Authors: Qing-Bing Wang
Yu Han
Ting-Ting Jiang
Wei-Min Chai
Ke-Min Chen
Bing-Ya Liu
Li-Fu Wang
Chunfu Zhang
Deng-Bin Wang
Publication date: 01-05-2011
Publisher: Springer-Verlag
Published in: European Radiology / Issue 5/2011
Print ISSN: 0938-7994
Electronic ISSN: 1432-1084
DOI: https://doi.org/10.1007/s00330-010-1988-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MR Imaging of activated hepatic stellate cells in liver injured by CCl₄ of rats with integrin-targeted ultrasmall superparamagnetic iron oxide

Abstract

Objective

Materials and Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Materials and Methods

Results

Conclusion

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