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Journal of Assisted Reproduction and Genetics

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01-02-2017 | Embryo Biology

Morphokinetics of vitrified and warmed blastocysts predicts implantation potential

Authors: T. Ebner, P. Oppelt, E. Radler, C. Allerstorfer, A. Habelsberger, R. B. Mayer, O. Shebl

Published in: Journal of Assisted Reproduction and Genetics | Issue 2/2017

Abstract

Purpose

It was studied whether morphokinetics of blastocoele re-expansion and hatching in vitrified-warmed blastocysts is predictive of implantation, clinical pregnancy, and live birth.

Methods

In 144 patients aiming for single warmed blastocyst transfer, blastocysts were cultured in a new time-lapse system (Miri® TL) immediately after warming. Video sequences with an image interval of 5 min were annotated and the corresponding morphokinetic variables were correlated with pregnancy outcome. In detail, tRE (start of re-expansion), tCRE (completion of re-expansion), tAH (hatching from the manipulated zona pellucida), and presence of collapses were recorded.

Results

In the pregnant group, tRE and tCRE were significantly lower (0.69 ± 0.45 h and 2.16 ± 0.94 h) as compared to the non-pregnant group (1.23 ± 1.08 h and 2.70 ± 1.20 h). Both variables and the duration of re-expansion (tCRE-tRE) allowed for distinction between “non-pregnant,” “loss of pregnancy,” and live birth/ongoing pregnancy. Presence and number of collapses showed no correlation with outcome.

Conclusions

Time-lapse imaging of vitrified-warmed blastocysts offers additional selection criteria allowing for prediction of implantation potential. As a consequence, cumulative pregnancy rate could be increased and time-to-pregnancy reduced.

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Title: Morphokinetics of vitrified and warmed blastocysts predicts implantation potential
Authors: T. Ebner
P. Oppelt
E. Radler
C. Allerstorfer
A. Habelsberger
R. B. Mayer
O. Shebl
Publication date: 01-02-2017
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2/2017
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-016-0855-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Morphokinetics of vitrified and warmed blastocysts predicts implantation potential

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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