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Molecular Imaging and Biology

Published in:

01-04-2012 | ResearcH Article

Molecular Imaging of Cell Death in an Experimental Model of Parkinson’s Disease with a Novel Apoptosis-Targeting Peptide

Authors: Min-Jeong Lee, Kai Wang, In-San Kim, Byung-Heon Lee, Hyung Soo Han

Published in: Molecular Imaging and Biology | Issue 2/2012

Abstract

Purpose

We used a novel apoptosis-targeting peptide called ApoPep-1 in order to evaluate whether ApoPep-1 can be used as a diagnostic indicator in a model of Parkinson’s disease (PD).

Procedures

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was given to mice to produce the PD model. Cy7.5-labeled ApoPep-1 was given intravenously and optical imaging was taken at 1, 2, and 3 weeks after MPTP treatment. Immunohistochemical study was performed with brain sections.

Results

Increased ApoPep-1 signal was observed in the brain of MPTP-treated mice by in vivo and ex vivo imaging study. With histological evaluation, ApoPep-1 signal demonstrated a strong correlation with loss of dopaminergic neurons or increase of apoptotic cells. Moreover, the neuroprotective effect of amantadine in the MPTP model was effectively evaluated using optical imaging of ApoPep-1.

Conclusions

We conclude that ApoPep-1 is the effective probe for imaging of apoptosis in the MPTP model and can be applied in brain diseases with apoptosis.

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Title: Molecular Imaging of Cell Death in an Experimental Model of Parkinson’s Disease with a Novel Apoptosis-Targeting Peptide
Authors: Min-Jeong Lee
Kai Wang
In-San Kim
Byung-Heon Lee
Hyung Soo Han
Publication date: 01-04-2012
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 2/2012
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-011-0497-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Molecular Imaging of Cell Death in an Experimental Model of Parkinson’s Disease with a Novel Apoptosis-Targeting Peptide

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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