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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2007

Open Access 01-12-2007 | Research article

Molecular epidemiology of Hepatitis B virus genotypes in Pakistan

Authors: Muhammad Masroor Alam, Sohail Zahoor Zaidi, Salman Akbar Malik, Shahzad Shaukat, Asif Naeem, Salmaan Sharif, Mehar Angez, Javed Aslam Butt

Published in: BMC Infectious Diseases | Issue 1/2007

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Abstract

Background

Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population.

Methods

A total of 690 individuals were enrolled for HBV screening with EIA and nested PCR. Positive samples were further analyzed to determine HBV genotypes (A-F) by multiplex-PCR using type specific primers.

Results

110 (15.94%) individuals were positive for HBV, including 64% males and 36% females. Out of these, 66 samples (65.34%) were classified into genotype D, 27 (26.73%) were of genotype B while 5(4.95%) had genotype A. In 3 (2.98%) samples, multiple genotypes were detected (genotype A+B; 2(1.99%) and genotypes B+D; 1(0.99%). Nine (8.18%) samples remained untyable.

Conclusion

In Asia, genotypes B and C are the most prevalent but our study reveals that genotype D is predominant and HBV infection constitutes a significant health problem in Pakistan.
Appendix
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Metadata
Title
Molecular epidemiology of Hepatitis B virus genotypes in Pakistan
Authors
Muhammad Masroor Alam
Sohail Zahoor Zaidi
Salman Akbar Malik
Shahzad Shaukat
Asif Naeem
Salmaan Sharif
Mehar Angez
Javed Aslam Butt
Publication date
01-12-2007
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2007
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-7-115

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