Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2013

Open Access 01-12-2013 | Research article

Modulation of experimental atopic dermatitis by topical application of Gami-Cheongyeul-Sodok-Eum

Authors: Ji Sun Hwang, Jung-Eun Kim, Young-Beob Yu, Sin-Hyeog Im

Published in: BMC Complementary Medicine and Therapies | Issue 1/2013

Login to get access

Abstract

Background

Gami-Cheongyeul-Sodok-Eum (GCSE), an herbal formula of traditional Korean medicine, comprises nine herb components. GCSE has various biological activities such as anti-inflammatory, anti-bacterial and anti-viral activities. However, it is still unclear whether GCSE has any immunomodulatory effect on atopic dermatitis (AD).

Methods

GCSE was treated to primary B cells and CD4+ T cells isolated from atopic mice to compare its inhibitory effects on IgE secretion and cytokine expression. Experimental AD was established by alternative treatment of 2, 4-dinitrochlorobenzene (DNCB) and house dust mite extract to the ears of BALB/c mice. GCSE was topically applied to ears of atopic mice every day for 3 weeks. AD progression was analyzed by measuring ear thickness, serum IgE level, histological examination of ear tissue by H&E staining and cytokine profile of CD4+ T cells and CD19+ B cells by real time PCR and ELISA.

Results

Treatment of GCSE significantly reduced IgE production and expression of AD associated pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13, IL-17, TNF-α, and IFN-γ by lymphocytes isolated from AD-induced mice. Topical application of GCSE on the ears of AD-induced mice significantly reduced ear thickness, clinical score and lymphocytes infiltration to ears as compared to control group. GCSE treatment also reduced serum IgE level and the levels of major pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13 and IL-17. In addition, GCSE treatment significantly increased Foxp3 expression level.

Conclusions

The protective effect of GCSE in experimental AD is mediated by inhibition of IgE production, by reduction in the levels of pathogenic cytokines and by induction of Foxp3, all of which are suggesting the beneficial effect of GCSE on modulating atopic dermatitis.
Appendix
Available only for authorised users
Literature
1.
Leung DYM, Jain N, Leo HL: New concepts in the pathogenesis of atopic dermatitis. Curr Opin Immunol. 2003, 15 (6): 634-638. 10.1016/j.coi.2003.09.009.CrossRefPubMed
2.
Boguniewicz M, Leung DYM: Recent insights into atopic dermatitis and implications for management of infectious complications. J Allergy Clin Immunol. 2011, 125 (1): 4-13.CrossRef
3.
Kupper TS, Fuhlbrigge RC: Immune surveillance in the skin: mechanisms and clinical consequences. Nat Rev Immunol. 2004, 4 (3): 211-222. 10.1038/nri1310.CrossRefPubMed
4.
De Benedetto A, Agnihothri R, McGirt LY, Bankova LG, Beck LA: Atopic dermatitis: a disease caused by innate immune defects [quest]. J Invest Dermatol. 2009, 129 (1): 14-30. 10.1038/jid.2008.259.CrossRefPubMed
5.
Leung DYM: Atopic dermatitis: The skin as a window into the pathogenesis of chronic allergic diseases. J Allergy Clin Immunol. 1995, 96 (3): 302-319. 10.1016/S0091-6749(95)70049-8.CrossRefPubMed
6.
Chen L, Martinez O, Overbergh L, Mathieu C, Prabhakar BS, Chan LS: Early up-regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model. Clin Exp Immunol. 2004, 138 (3): 375-387. 10.1111/j.1365-2249.2004.02649.x.CrossRefPubMedPubMedCentral
7.
Lee H-J, Lee H-P, Ha S-J, Byun D-G, Kim J-W: Spontaneous expression of mRNA for IL-10, GM-CSF, TGF-[beta], TNF-[alpha], and IL-6 in peripheral blood mononuclear cells from atopic dermatitis. Ann Allergy Asthma Immunol. 2000, 84 (5): 553-558. 10.1016/S1081-1206(10)62522-7.CrossRefPubMed
8.
Tazawa T, Sugiura H, Sugiura Y, Uehara M: Relative importance of IL-4 and IL-13 in lesional skin of atopic dermatitis. Arch Dermatol Res. 2004, 295 (11): 459-464. 10.1007/s00403-004-0455-6.CrossRefPubMed
9.
Bruynzeel-Koomen C, Wichen DF, Toonstra J, Berrens L, Bruynzeel PLB: The presence of IgE molecules on epidermal langerhans cells in patients with atopic dermatitis. Arch Dermatol Res. 1986, 278 (3): 199-205. 10.1007/BF00412924.CrossRefPubMed
10.
Hajoui O, Janani R, Tulic M, Joubert P, Ronis T, Hamid Q, Zheng H, Mazer BD: Synthesis of IL-13 by human B lymphocytes: regulation and role in IgE production. J Allergy Clin Immunol. 2004, 114 (3): 657-663. 10.1016/j.jaci.2004.05.034.CrossRefPubMed
11.
Vestergaard C, Kirstejn N, Gesser B, Mortensen JT, Matsushima K, Larsen CG: IL-10 augments the IFN-[gamma] and TNF-[alpha] induced TARC production in HaCaT cells: a possible mechanism in the inflammatory reaction of atopic dermatitis. J Dermatol Sci. 2001, 26 (1): 46-54. 10.1016/S0923-1811(00)00160-2.CrossRefPubMed
12.
13.
Konur A, Schulz U, Eissner G, Andreesen R, Holler E: Interferon (IFN)-γ is a main mediator of keratinocyte (HaCaT) apoptosis and contributes to autocrine IFN-γ and tumour necrosis factor-α production. Br J Dermatol. 2005, 152 (6): 1134-1142. 10.1111/j.1365-2133.2005.06508.x.CrossRefPubMed
14.
Richardson MA, Sanders T, Palmer JL, Greisinger A, Singletary SE: Complementary/alternative medicine use in a comprehensive cancer center and the implications for oncology. J Clin Oncol. 2000, 18 (13): 2505-2514.PubMed
15.
Lee K-H: Research and future trends in the pharmaceutical development of medicinal herbs from Chinese medicine. Public Health Nutr. 2000, 3 (4a): 515-522.CrossRefPubMed
16.
Jeon B-H JW-Y: Influence of medicinal herba drug on the experimental model of thrombosis in animal. Kor J Orient Med Pathol. 1996, 10 (1): 72-78.
17.
Kim J-J KH-T: Effect of Chongyeal-sodok-yeum on chemokines expression in lung epithelial cells. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2006, 19 (1): 115-122.
18.
Kim YW, Zhao RJ, Park SJ, Lee JR, Cho IJ, Yang CH, Kim SG, Kim SC: Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-κB-dependent iNOS and proinflammatory cytokines production. Br J Pharmacol. 2008, 154 (1): 165-173. 10.1038/bjp.2008.79.CrossRefPubMedPubMedCentral
19.
Kim J-H, Jeong J-H, Jeon S-T, Kim H, Ock J, Suk K, Kim S-I, Song K-S, Lee W-h: Decursin inhibits induction of inflammatory mediators by blocking nuclear factor-kB activation in macrophages. Mol Pharmacol. 2006, 69 (6): 1783-1790. 10.1124/mol.105.021048.CrossRefPubMed
20.
Ryu M, Kim EH, Chun M, Kang S, Shim B, Yu Y-B, Jeong G, Lee J-S: Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk. J Ethnopharmacol. 2008, 115 (2): 184-193. 10.1016/j.jep.2007.09.027.CrossRefPubMed
21.
Kwon H-K, Lee C-G, So J-S, Chae C-S, Hwang J-S, Sahoo A, Nam JH, Rhee JH, Hwang K-C, Im S-H: Generation of regulatory dendritic cells and CD4 + Foxp3+ T cells by probiotics administration suppresses immune disorders. Proc Natl Acad Sci. 2010, 107 (5): 2159-2164. 10.1073/pnas.0904055107.CrossRefPubMedPubMedCentral
22.
Hwang JS, Kwon H-K, Kim J-E, Rho J, Im S-H: Immunomodulatory effect of water soluble extract separated from mycelium of Phellinus linteus on experimental atopic dermatitis. BMC Complement Altern Med. 2012, 12 (1): 159-10.1186/1472-6882-12-159.CrossRefPubMedPubMedCentral
23.
Homey B, Steinhoff M, Ruzicka T, Leung DYM: Cytokines and chemokines orchestrate atopic skin inflammation. J Allergy Clin Immunol. 2006, 118 (1): 178-189. 10.1016/j.jaci.2006.03.047.CrossRefPubMed
24.
Son JS, Chae CS, Hwang JS, Park ZY, Im SH: Enhanced chromatin accessibility and recruitment of JUNB mediate the sustained IL-4 expression in NFAT1 deficient T helper 2 cells. PLoS One. 2011, 6 (7): e22042-10.1371/journal.pone.0022042.CrossRefPubMedPubMedCentral
25.
Hwang JS, Kim JE, Kim HT, Yu YB, Im SH: Topical application of Taglisodog-eum inhibits the development of experimental atopic dermatitis. J Ethnopharmacol. 2012, 145 (2): 536-546.CrossRefPubMed
26.
M-J O, Yoo Y-B, Lim S-H, Kim H-T: A clinical study for the effect of ointment contained Takrisodok-Eum on recovering the damaged skin barrier by Atopic dermatitis. J Korean Orient Med Ophthalmol Otolaryngol Dermatol. 2011, 24 (3): 84-107.
27.
An B-J LJ-T, Lee C-E, Son J-H, Lee J-Y, Park T-S, Lee I-C, Song M-A, Cheon S-J, Jee S-Y: Cytotoxicity, antibacterial and antioxidant activities of the prescription Cheongyeolsodokum and its constituent herbs. Kor J Herbology. 2005, 20 (4): 41-51.
28.
H-M K: Effect of Chongyealsodokyeum on the experimental inflammation and thrombosis. J Orient Med Pathol. 1995, 10: 267-293.
29.
Schröfelbauer BRJ, Hauner M, Wolkerstorfer A, Ernst W, Szolar OHJ: Glycyrrhizin, the main active compound in liquorice, attenuates pro-inflammatory responses by interfering with membrane-dependent receptor signalling. Biochem J. 2009, 421 (3): 473-482. 10.1042/BJ20082416.CrossRefPubMed
30.
Kang HS, Lee JY, Kim CJ: Anti-inflammatory activity of arctigenin from Forsythiae Fructus. J Ethnopharmacol. 2008, 116 (2): 305-312. 10.1016/j.jep.2007.11.030.CrossRefPubMed
31.
Akdis CA, Akdis M, Bieber T, Bindslev-Jensen C, Boguniewicz M, Eigenmann P, Hamid Q, Kapp A, Leung DYM, Lipozencic J: Diagnosis and treatment of atopic dermatitis in children and adults: European Academy of Allergology and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL Consensus Report. J Allergy Clin Immunol. 2006, 118 (1): 152-169. 10.1016/j.jaci.2006.03.045.CrossRefPubMed
32.
Cookson W: The immunogenetics of asthma and eczema: a new focus on the epithelium. Nat Rev Immunol. 2004, 4 (12): 978-988. 10.1038/nri1500.CrossRefPubMed
33.
Wollenberg A, Bieber T: Atopic dermatitis: from the genes to skin lesions. Allergy. 2000, 55 (3): 205-213. 10.1034/j.1398-9995.2000.00115.x.CrossRefPubMed
34.
Padilla J, Daley E, Chow A, Robinson K, Parthasarathi K, McKenzie ANJ, Tschernig T, Kurup VP, Donaldson DD, Grunig G: IL-13 Regulates the Immune Response to Inhaled Antigens. J Immunol. 2005, 174 (12): 8097-8105.CrossRefPubMed
35.
Fujisawa T, Fujisawa R, Kato Y, Nakayama T, Morita A, Katsumata H, Nishimori H, Iguchi K, Kamiya H, Gray PW: Presence of high contents of thymus and activation-regulated chemokine in platelets and elevated plasma levels of thymus and activation-regulated chemokine and macrophage-derived chemokine in patients with atopic dermatitis. J Allergy Clin Immunol. 2002, 110 (1): 139-146. 10.1067/mai.2002.126079.CrossRefPubMed
36.
Jahnz-Rozyk K, Targowski T, Paluchowska E, Owczarek W, Kucharczyk A: Serum thymus and activation-regulated chemokine, macrophage-derived chemokine and eotaxin as markers of severity of atopic dermatitis. Allergy. 2005, 60 (5): 685-688. 10.1111/j.1398-9995.2005.00774.x.CrossRefPubMed
37.
38.
Nakae S, Komiyama Y, Nambu A, Sudo K, Iwase M, Homma I, Sekikawa K, Asano M, Iwakura Y: Antigen-specific T cell sensitization is impaired in IL-17-deficient mice, causing suppression of allergic cellular and humoral responses. Immunity. 2002, 17 (3): 375-387. 10.1016/S1074-7613(02)00391-6.CrossRefPubMed
39.
Wills-Karp M, Luyimbazi J, Xu X, Schofield B, Neben TY, Karp CL, Donaldson DD: Interleukin-13: Central mediator of allergic asthma. Science. 1998, 282 (5397): 2258-2261.CrossRefPubMed
40.
Kabesch M, Schedel M, Carr D, Woitsch B, Fritzsch C, Weiland SK, von Mutius E: IL-4/IL-13 pathway genetics strongly influence serum IgE levels and childhood asthma. J Allergy Clin Immunol. 2006, 117 (2): 269-274. 10.1016/j.jaci.2005.10.024.CrossRefPubMed
41.
Ziegler SF: FOXP3: of mice and men. Annu Rev Immunol. 2006, 24 (1): 209-226. 10.1146/annurev.immunol.24.021605.090547.CrossRefPubMed
Metadata
Title
Modulation of experimental atopic dermatitis by topical application of Gami-Cheongyeul-Sodok-Eum
Authors
Ji Sun Hwang
Jung-Eun Kim
Young-Beob Yu
Sin-Hyeog Im
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2013
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-13-312

Other articles of this Issue 1/2013

BMC Complementary Medicine and Therapies 1/2013 Go to the issue

Research article

Anti-HIV-1 activity, protease inhibition and safety profile of extracts prepared from Rhus parviflora

Research article

Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats

Research article

Extract of Scutellaria baicalensis inhibits dengue virus replication

Research article

A survey of Canadian regulated complementary and alternative medicine schools about research, evidence-based health care and interprofessional training, as well as continuing education

Research article

Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice

Research article

Prokinetic and laxative effects of the crude methanolic extract of Viola betonicifolia whole plant in rodents