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01-06-2012 | Basic Neurosciences, Genetics and Immunology - Original Article

MK-801 alters Na⁺, K⁺-ATPase activity and oxidative status in zebrafish brain: reversal by antipsychotic drugs

Authors: Kelly Juliana Seibt, Renata da Luz Oliveira, Denis Broock Rosemberg, Luiz Eduardo Baggio Savio, Emilene B. S. Scherer, Felipe Schmitz, Angela T. S. Wyse, Carla Denise Bonan

Published in: Journal of Neural Transmission | Issue 6/2012

Abstract

Schizophrenia is a debilitating mental disorder with a global prevalence of 1% and its etiology remains poorly understood. In the current study we investigated the influence of antipsychotic drugs on the effects of MK-801 administration, which is a drug that mimics biochemical changes observed in schizophrenia, on Na⁺, K⁺-ATPase activity and some parameters of oxidative stress in zebrafish brain. Our results showed that MK-801 treatment significantly decreased Na⁺, K⁺-ATPase activity, and all antipsychotics tested prevented such effects. Acute MK-801 treatment did not alter reactive oxygen/nitrogen species by 2′7′-dichlorofluorscein (H2DCF) oxidation assay, but increased the levels of thiobarbituric acid reactive substances (TBARS), when compared with controls. Some antipsychotics such as sulpiride, olanzapine, and haloperidol prevented the increase of TBARS caused by MK-801. These findings indicate oxidative damage might be a mechanism involved in the decrease of Na⁺, K⁺-ATPase activity induced by MK-801. The parameters evaluated in this study had not yet been tested in this animal model using the MK-801, suggesting that zebrafish is an animal model that can contribute for providing information on potential treatments and disease characteristics.

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Title: MK-801 alters Na+, K+-ATPase activity and oxidative status in zebrafish brain: reversal by antipsychotic drugs
Authors: Kelly Juliana Seibt
Renata da Luz Oliveira
Denis Broock Rosemberg
Luiz Eduardo Baggio Savio
Emilene B. S. Scherer
Felipe Schmitz
Angela T. S. Wyse
Carla Denise Bonan
Publication date: 01-06-2012
Publisher: Springer Vienna
Published in: Journal of Neural Transmission / Issue 6/2012
Print ISSN: 0300-9564
Electronic ISSN: 1435-1463
DOI: https://doi.org/10.1007/s00702-011-0745-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MK-801 alters Na⁺, K⁺-ATPase activity and oxidative status in zebrafish brain: reversal by antipsychotic drugs

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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