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Discover Oncology
Published in: Discover Oncology 1/2023

Open Access 01-12-2023 | Research

MiR-3529-3p from PDGF-BB-induced cancer-associated fibroblast-derived exosomes promotes the malignancy of oral squamous cell carcinoma

Authors: Dingyun You, Yanghao Wang, Jianguo Xu, Rongqiang Yang, Weizhou Wang, Xiaofang Wang, Xue Cao, Yiting Li, Lifu Yu, Weihong Wang, Yanan Shi, Changbin Zhang, Hefeng Yang, Yongwen He, Li Bian

Published in: Discover Oncology | Issue 1/2023

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Abstract

Aims

This study aims to explore the role of exosomes from cancer-associated fibroblasts (CAFs) induced by PDGF-BB in promoting the malignancy of oral squamous cell carcinoma (OSCC) and provide new insight into the mechanism of OSCC progression and its treatment.

Main methods

Exosomes were extracted from human oral mucosa fibroblasts (hOMFs) and CAFs. Differentially expressed miRNAs of exosomes between hOMFs and CAFs were analysed using high-throughput sequencing and self-programmed R software. Cal-27, a human tongue squamous carcinoma cell line, was treated with exosomes. Differentially expressed miRNAs between clinical cancer tissues and adjacent tissues and between hOMF and CAF exosomes were verified by qRT‒PCR. The effect of miR-3529-3p on Cal-27 cells was clarified by overexpressing or knocking down miR-3529-3p in Cal-27 cells. Sample expression and differentially expressed miRNA expression were compared between cancer and paracarcinoma tissues.

Key findings

We found that exosomes from CAFs (CAF-Exos) were internalized by tongue squamous carcinoma cells and promoted their proliferation, migration, invasion, and antiapoptotic effects. MiR-3529-3p was a significant differentially expressed miRNA between CAF-Exos and exosomes from hOMFs (hOMF-Exos). The overexpression of miR-3529-3p promoted proliferation, migration, and invasion and inhibited apoptosis of Cal-27 cells.

Significance

This study explores the role of PDGF-BB-induced CAFs in promoting malignancy in OSCC. This study will provide new insight into the mechanism of OSCC progression and its treatment.
Appendix
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Metadata
Title
MiR-3529-3p from PDGF-BB-induced cancer-associated fibroblast-derived exosomes promotes the malignancy of oral squamous cell carcinoma
Authors
Dingyun You
Yanghao Wang
Jianguo Xu
Rongqiang Yang
Weizhou Wang
Xiaofang Wang
Xue Cao
Yiting Li
Lifu Yu
Weihong Wang
Yanan Shi
Changbin Zhang
Hefeng Yang
Yongwen He
Li Bian
Publication date
01-12-2023
Publisher
Springer US
Published in
Discover Oncology / Issue 1/2023
Print ISSN: 1868-8497
Electronic ISSN: 2730-6011
DOI
https://doi.org/10.1007/s12672-023-00753-9

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