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Diagnostic Pathology

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Open Access 01-12-2015 | Research

MiR-186 suppresses the growth and metastasis of bladder cancer by targeting NSBP1

Authors: Kun Yao, Leye He, Yu Gan, Qing Zeng, Yingbo Dai, Jing Tan

Published in: Diagnostic Pathology | Issue 1/2015

Abstract

Background

Increasing evidence has shown that microRNAs function as oncogenes or tumor suppressors in human malignancies, but the roles of miR-186 in human bladder cancer (BC) is still unclear.

Methods

First, quantitative real-time PCR (qRT-PCR) was performed to detect miR-186 expression in bladder cancer tissues and cell lines. Then, Bioinformatics analysis, combined with luciferase reporter assay demonstrated the target gene of miR-186. Finally, the roles of miR-186 in regulation of tumor proliferation and invasion were further investigated.

Results

Here, our study showed miR-186 was down-regulated in bladder cancer tissues and cell lines. Luciferase reporter assay showed that miR-186 targets NSBP1 3′-untranslated region (UTR) directly and suppresses NSBP1 (HMGN5) expression in human bladder cancer cells. NSBP1 siRNA- and miR-186-mediated NSBP1 knock-down experiments revealed that miR-186 suppresses cell proliferation and invasion through suppression of NSBP1 expression. Expression analysis of a set of epithelial-mesenchymal transition (EMT) markers showed that NSBP1 involves miR-186 suppressed EMT which reducing the expression of mesenchymal markers (vimentin and N-cadherin) and inducing the expression of epithelial marker (E-cadherin).

Conclusions

Our data first time identified miR-186 as the upstream regulator of NSBP1 and also suggest miR-186-suppressed NSBP1 as a novel therapeutic approach for bladder cancer.

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Title: MiR-186 suppresses the growth and metastasis of bladder cancer by targeting NSBP1
Authors: Kun Yao
Leye He
Yu Gan
Qing Zeng
Yingbo Dai
Jing Tan
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2015
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-015-0372-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MiR-186 suppresses the growth and metastasis of bladder cancer by targeting NSBP1

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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