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01-05-2017 | Original Article

MiR-142-5p promotes bone repair by maintaining osteoblast activity

Authors: Manli Tu, Juanjuan Tang, Hongbo He, Peng Cheng, Chao Chen

Published in: Journal of Bone and Mineral Metabolism | Issue 3/2017

Abstract

MicroRNAs play important roles in regulating bone regeneration and remodeling. However, the pathophysiological roles of microRNAs in bone repair remain unclear. Here we identify a significant upregulation of miR-142-5p correlated with active osteoblastogenesis during the bone healing process. In vitro, miR-142-5p promoted osteoblast activity and matrix mineralization by targeting the gene encoding WW-domain-containing E3 ubiquitin protein ligase 1. We also found that the expression of miR-142-5p in the callus of aged mice was lower than that in the callus of young mice and directly correlated with the age-related delay in bone healing. Furthermore, treatment with agomir-142-5p in the fracture areas stimulated osteoblast activity which repaired the bone fractures in aged mice. Thus, our study revealed that miR-142-5p plays a crucial role in healing fractures by maintaining osteoblast activity, and provided a new molecular target therapeutic strategy for bone healing.

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Title: MiR-142-5p promotes bone repair by maintaining osteoblast activity
Authors: Manli Tu
Juanjuan Tang
Hongbo He
Peng Cheng
Chao Chen
Publication date: 01-05-2017
Publisher: Springer Japan
Published in: Journal of Bone and Mineral Metabolism / Issue 3/2017
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-016-0757-8

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