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BMC Cancer
Published in: BMC Cancer 1/2016

Open Access 01-12-2016 | Research article

miR-124-3p functions as a tumor suppressor in breast cancer by targeting CBL

Authors: Yanbo Wang, Luxiao Chen, Zhenyu Wu, Minghai Wang, Fangfang Jin, Nan Wang, Xiuting Hu, Zhengya Liu, Chen-Yu Zhang, Ke Zen, Jiangning Chen, Hongwei Liang, Yujing Zhang, Xi Chen

Published in: BMC Cancer | Issue 1/2016

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Abstract

Background

The origin and development of breast cancer remain complex and obscure. Recently, microRNA (miRNA) has been identified as an important regulator of the initiation and progression of breast cancer, and some studies have shown the essential role of miR-124-3p as a tumor suppressor in breast tumorigenesis. However, the detailed role of miR-124-3p in breast cancer remains poorly understood.

Methods

Quantitative RT-PCR and western blotting assays were used to measure miR-124-3p and CBL expression levels in breast cancer tissues, respectively. Luciferase reporter assay was employed to validate the direct targeting of CBL by miR-124-3p. Cell proliferation and invasion assays were performed to analyze the biological functions of miR-124-3p and CBL in breast cancer cells.

Results

In the present study, we found that miR-124-3p was consistently downregulated in breast cancer tissues. Moreover, we showed that miR-124-3p significantly suppressed the proliferation and invasion of breast cancer cells. In addition, we investigated the molecular mechanism through which miR-124-3p contributes to breast cancer tumorigenesis and identified CBL (Cbl proto-oncogene, E3 ubiquitin protein ligase) as a direct target gene of miR-124-3p. Moreover, we found that ectopic expression of CBL can attenuate the inhibitory effect of miR-124-3p on cell proliferation and invasion in breast cancer cells.

Conclusions

This study identified a new regulatory axis in which miR-124-3p and CBL regulate the proliferation and invasion of breast cancer cells.
Appendix
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Metadata
Title
miR-124-3p functions as a tumor suppressor in breast cancer by targeting CBL
Authors
Yanbo Wang
Luxiao Chen
Zhenyu Wu
Minghai Wang
Fangfang Jin
Nan Wang
Xiuting Hu
Zhengya Liu
Chen-Yu Zhang
Ke Zen
Jiangning Chen
Hongwei Liang
Yujing Zhang
Xi Chen
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2016
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/s12885-016-2862-4

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