Top

Published in:

01-04-2019 | Original Article

Mineral density changes in bovine carious dentin after treatment with bioactive dental cements: a comparative micro-CT study

Authors: Aline Borburema Neves, Tamiris Gomes Bergstrom, Andréa Fonseca-Gonçalves, Thais Maria Pires dos Santos, Ricardo Tadeu Lopes, Aline de Almeida Neves

Published in: Clinical Oral Investigations | Issue 4/2019

Abstract

Objectives

To evaluate the potential of conventional glass ionomer cement (GIC), Biodentine™, MTA, and Portland cement to induce mineral density changes in carious dentin compared to zinc oxide eugenol control cement (ZOE).

Materials and methods

Fifty blocks of bovine root dentin were prepared and a biofilm model using ATCC strains of S.mutans, S.sobrinus, and L.casei was used to promote artificial dentin lesions. After demineralization, the blocks were randomly divided into the five cement groups. Half of the surface of each specimen received the tested material and the other half was covered with wax (control). Samples were stored in phosphate buffered saline solution for 30 days and after that were scanned in a micro-CT with standardized parameters. Dentin mineral density changes were calculated using differences in plot profiles of the exposed and control carious dentin. Friedman’s test, followed by Wilcoxon signed-rank test was used with 5% significance.

Results

Mean ΔZ values for the cements were 48.63 ± 19.09 for the control (ZOE), 63.31 ± 32.59 for Biodentine™, 114.63 ± 72.92 for GIC, 109.56 ± 66.28 for MTA, and 106.88 ± 66.02 for Portland cement. All cements showed a statistically significant increase in ΔZ values compared to the control, but Biodentine™ values were statistically significantly lower compared to GIC and the other calcium silicate cements.

Conclusions

Tested materials present potential to induce mineral density changes in carious bovine dentin. MTA, Portland, and GIC showed higher bioactivity potential than Biodentine™.

Clinical relevance

Based on minimally invasive concept, materials with remineralization potential can be used to preserve diseased but still repairable dental tissue.

Banerjee A (2013) Minimal intervention dentistry: part 7. Minimally invasive operative caries management: rationale and techniques. Br Dent J 214(3):107–111. https://doi.org/10.1038/sj.bdj.2013.106 CrossRefPubMed

Dai L, Liu Y, Salameh Z, Khan S, Mao J, Pashley DH, Tay FR (2011) Can caries-affected dentin be completely remineralized by guided tissue remineralization? Dent Hypotheses 2(2):74–82. https://doi.org/10.5436/j.dehy.2010.1.00011

Hamama HH, Yiu CK, Burrow MF (2015) Caries management: a journey between Black’s principals and minimally invasive concepts. Int J Dent Oral Sci 2(8):120–125

Watson TF, Atmeh AR, Sajini S, Cook RJ, Festy F (2014) Present and future of glass-ionomers and calcium-silicate cements as bioactive materials in dentistry: biophotonics-based interfacial analyses in health and disease. Dent Mater 30(1):50–61. https://doi.org/10.1016/j.dental.2013.08.202 CrossRefPubMedPubMedCentral

Vallittu PK, Boccaccini AR, Hupa L, Watts DC (2018) Bioactive dental materials-do they exist and what does bioactivity mean? Dent Mater 34(5):693–694. https://doi.org/10.1016/j.dental.2018.03.001 CrossRefPubMed

Goldberg M, Kulkarni AB, Young M, Boskey A (2011) Dentin: structure, composition and mineralization. Front Biosci 3:711–735CrossRef

Liu Y, Li N, Qi Y, Niu LN, Elshafiy S, Mao J, Breschi L, Pashley DH, Tay FR (2011) The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin. Dent Mater 27(5):465–477. https://doi.org/10.1016/j.dental.2011.01.008 CrossRefPubMedPubMedCentral

Koc Vural U, Kiremitci A, Gokalp S (2017) Randomized clinical trial to evaluate MTA indirect pulp capping in deep caries lesions after 24-months. Oper Dent 42(5):470–477. https://doi.org/10.2341/16-110-C CrossRefPubMed

Kind L, Stevanovic S, Wuttig S, Wimberger S, Hofer J, Muller B, Pieles U (2017) Biomimetic remineralization of carious lesions by self-assembling peptide. J Dent Res 96(7):790–797. https://doi.org/10.1177/0022034517698419 CrossRefPubMed

10.

Okamoto M, Takahashi Y, Komichi S, Ali M, Yoneda N, Ishimoto T, Nakano T, Hayashi M (2018) Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography. Clin Oral Investig. https://doi.org/10.1007/s00784-018-2374-5

11.

Han L, Okiji T (2011) Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J 44(12):1081–1087. https://doi.org/10.1111/j.1365-2591.2011.01924.x CrossRefPubMed

12.

Laird NM, Mosteller F (1990) Some statistical methods for combining experimental results. Int J Technol Assess Health Care 6(1):5–30CrossRef

13.

Cohen J (1992) A power primer. Psychol Bull 112(1):155–159CrossRef

14.

Niu LN, Zhang W, Pashley DH, Breschi L, Mao J, Chen JH, Tay FR (2014) Biomimetic remineralization of dentin. Dent Mater 30(1):77–96. https://doi.org/10.1016/j.dental.2013.07.013 CrossRefPubMed

15.

Camilleri J, Sorrentino F, Damidot D (2013) Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater 29(5):580–593. https://doi.org/10.1016/j.dental.2013.03.007 CrossRefPubMed

16.

Niu LN, Jiao K, Wang TD, Zhang W, Camilleri J, Bergeron BE, Feng HL, Mao J, Chen JH, Pashley DH, Tay FR (2014) A review of the bioactivity of hydraulic calcium silicate cements. J Dent 42(5):517–533. https://doi.org/10.1016/j.jdent.2013.12.015 CrossRefPubMedPubMedCentral

17.

Reyes-Carmona JF, Felippe MS, Felippe WT (2009) Biomineralization ability and interaction of mineral trioxide aggregate and white Portland cement with dentin in a phosphate-containing fluid. J Endod 35(5):731–736. https://doi.org/10.1016/j.joen.2009.02.011 CrossRefPubMed

18.

Simila HO, Karpukhina N, Hill RG (2018) Bioactivity and fluoride release of strontium and fluoride modified Biodentine. Dent Mater 34(1):e1–e7. https://doi.org/10.1016/j.dental.2017.10.005 CrossRefPubMed

19.

Pires PM, Santos TP, Fonseca-Goncalves A, Pithon MM, Lopes RT, Neves AA (2018) Mineral density in carious dentine after treatment with calcium silicates and polyacrylic acid based cements. Int Endod J. https://doi.org/10.1111/iej.12941

20.

Corral Nunez C, Covarrubias C, Fernandez E, Oliveira OBJ (2017) Enhanced bioactive properties of BiodentineTM modified with bioactive glass nanoparticles. J Appl Oral Sci 25(2):177–185. https://doi.org/10.1590/1678-77572016-0209 CrossRefPubMedPubMedCentral

21.

Ngo HC, Mount G, Mc Intyre J, Tuisuva J, Von Doussa RJ (2006) Chemical exchange between glass-ionomer restorations and residual carious dentine in permanent molars: an in vivo study. J Dent 34(8):608–613. https://doi.org/10.1016/j.jdent.2005.12.012 CrossRefPubMed

22.

Baig MS, Dowling AH, Cao X, Fleming GJ (2015) A discriminatory mechanical testing performance indicator protocol for hand-mixed glass-ionomer restoratives. Dent Mater 31(3):273–283. https://doi.org/10.1016/j.dental.2014.12.012 CrossRefPubMed

23.

Amaral MT, Guedes-Pinto AC, Chevitarese O (2006) Effects of a glass-ionomer cement on the remineralization of occlusal caries--an in situ study. Braz Oral Res 20(2):91–96

24.

Beiruti N, Frencken JE, van ’t Hof MA, van Palenstein Helderman WH (2006) Caries-preventive effect of resin-based and glass ionomer sealants over time: a systematic review. Community Dent Oral Epidemiol 34(6):403–409. https://doi.org/10.1111/j.1600-0528.2006.00321.x CrossRefPubMed

25.

ten Cate JM, van Duinen RN (1995) Hypermineralization of dentinal lesions adjacent to glass-ionomer cement restorations. J Dent Res 74(6):1266–1271. https://doi.org/10.1177/00220345950740060501 CrossRefPubMed

26.

Kim YK, Yiu CK, Kim JR, Gu L, Kim SK, Weller RN, Pashley DH, Tay FR (2010) Failure of a glass ionomer to remineralize apatite-depleted dentin. J Dent Res 89(3):230–235. https://doi.org/10.1177/0022034509357172 CrossRefPubMedPubMedCentral

27.

Li X, De Munck J, Van Landuyt K, Pedano M, Chen Z, Van Meerbeek B (2017) How effectively do hydraulic calcium-silicate cements re-mineralize demineralized dentin. Dent Mater 33(4):434–445. https://doi.org/10.1016/j.dental.2017.01.015 CrossRefPubMed

28.

Malkondu O, Karapinar Kazandag M, Kazazoglu E (2014) A review on biodentine, a contemporary dentine replacement and repair material. Biomed Res Int 2014:160951–160910. https://doi.org/10.1155/2014/160951 CrossRefPubMedPubMedCentral

29.

Maske TT, van de Sande FH, Arthur RA, Huysmans M, Cenci MS (2017) In vitro biofilm models to study dental caries: a systematic review. Biofouling 33(8):661–675. https://doi.org/10.1080/08927014.2017.1354248 CrossRefPubMed

30.

Swain MV, Xue J (2009) State of the art of micro-CT applications in dental research. International J Oral Sci 1(4):177–188. https://doi.org/10.4248/IJOS09031

31.

Lee HS, Berg JH, Garcia-Godoy F, Jang KT (2008) Long-term evaluation of the remineralization of interproximal caries-like lesions adjacent to glass-ionomer restorations: a micro-CT study. Am J Dent 21(2):129–132PubMed

32.

Neves AA, Lourenco RA, Alves HD, Lopes RT, Primo LG (2015) Caries-removal effectiveness of a papain-based chemo-mechanical agent: a quantitative micro-CT study. Scanning 37(4):258–264. https://doi.org/10.1002/sca.21206 CrossRefPubMed

33.

Yassen GH, Platt JA, Hara AT (2011) Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci 53(3):273–282CrossRefPubMed

34.

Teruel Jde D, Alcolea A, Hernandez A, Ruiz AJ (2015) Comparison of chemical composition of enamel and dentine in human, bovine, porcine and ovine teeth. Arch Oral Biol 60(5):768–775. https://doi.org/10.1016/j.archoralbio.2015.01.014 CrossRefPubMed

Title: Mineral density changes in bovine carious dentin after treatment with bioactive dental cements: a comparative micro-CT study
Authors: Aline Borburema Neves
Tamiris Gomes Bergstrom
Andréa Fonseca-Gonçalves
Thais Maria Pires dos Santos
Ricardo Tadeu Lopes
Aline de Almeida Neves
Publication date: 01-04-2019
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 4/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2644-2

At a glance: The STEP trials

Springer Medicine

Mineral density changes in bovine carious dentin after treatment with bioactive dental cements: a comparative micro-CT study

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Please log in to get access to this content

Other articles of this Issue 4/2019

Changes in the facial soft-tissue profile after mandibular orthognathic surgery

Long-term outcomes of bimaxillary surgery for treatment of asymmetric skeletal class III deformity using surgery-first approach

Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis

Comparative study of all-ceramic crowns obtained from conventional and digital impressions: clinical findings

Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Correction to: Cytokine release kinetics of concentrated growth factors in different scaffolds