Published in:
Open Access 01-12-2017 | Preliminary research
Microglial depletion and activation: A [11C]PBR28 PET study in nonhuman primates
Published in: EJNMMI Research | Issue 1/2017
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Background
The 18-kDa translocator protein (TSPO) is an important target for assessing neuroimmune function in brain with positron-emission tomography (PET) imaging. The goal of this work was to assess two [11C]PBR28 imaging paradigms for measuring dynamic microglia changes in Macaca mulatta.
Methods
Dynamic [11C]PBR28 PET imaging data with arterial blood sampling were acquired to quantify TSPO levels as [11C]PBR28 V
T. Scans were acquired at three timepoints: baseline, immediately post-drug, and prolonged post-drug.
Results
In one animal, a colony-stimulating factor 1 receptor kinase inhibitor, previously shown to deplete brain microglia, reduced [11C]PBR28 V
T in brain by 46 ± 3% from baseline, which recovered after 12 days to 7 ± 5% from baseline. In a different animal, acute lipopolysaccharide administration, shown to activate brain microglia, increased [11C]PBR28 V
T in brain by 39 ± 9% from baseline, which recovered after 14 days to −11 ± 3% from baseline.
Conclusions
These studies provide preliminary evidence of complementary paradigms to assess microglia dynamics via in vivo TSPO imaging.