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Arthritis Research & Therapy
Published in: Arthritis Research & Therapy 2/2007

Open Access 01-04-2007 | Research article

Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes

Authors: Farida Djouad, Bruno Delorme, Marielle Maurice, Claire Bony, Florence Apparailly, Pascale Louis-Plence, François Canovas, Pierre Charbord, Danièle Noël, Christian Jorgensen

Published in: Arthritis Research & Therapy | Issue 2/2007

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Abstract

Chondrogenesis is a process involving stem-cell differentiation through the coordinated effects of growth/differentiation factors and extracellular matrix (ECM) components. Recently, mesenchymal stem cells (MSCs) were found within the cartilage, which constitutes a specific niche composed of ECM proteins with unique features. Therefore, we hypothesized that the induction of MSC differentiation towards chondrocytes might be induced and/or influenced by molecules from the microenvironment. Using microarray analysis, we previously identified genes that are regulated during MSC differentiation towards chondrocytes. In this study, we wanted to precisely assess the differential expression of genes associated with the microenvironment using a large-scale real-time PCR assay, according to the simultaneous detection of up to 384 mRNAs in one sample. Chondrogenesis of bone-marrow-derived human MSCs was induced by culture in micropellet for various periods of time. Total RNA was extracted and submitted to quantitative RT-PCR. We identified molecules already known to be involved in attachment and cell migration, including syndecans, glypicans, gelsolin, decorin, fibronectin, and type II, IX and XI collagens. Importantly, we detected the expression of molecules that were not previously associated with MSCs or chondrocytes, namely metalloproteases (MMP-7 and MMP-28), molecules of the connective tissue growth factor (CTGF); cef10/cyr61 and nov (CCN) family (CCN3 and CCN4), chemokines and their receptors chemokine CXC motif ligand (CXCL1), Fms-related tyrosine kinase 3 ligand (FlT3L), chemokine CC motif receptor (CCR3 and CCR4), molecules with A Disintegrin And Metalloproteinase domain (ADAM8, ADAM9, ADAM19, ADAM23, A Disintegrin And Metalloproteinase with thrombospondin type 1 motif ADAMTS-4 and ADAMTS-5), cadherins (4 and 13) and integrins (α4, α7 and β5). Our data suggest that crosstalk between ECM components of the microenvironment and MSCs within the cartilage is responsible for the differentiation of MSCs into chondrocytes.
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Metadata
Title
Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes
Authors
Farida Djouad
Bruno Delorme
Marielle Maurice
Claire Bony
Florence Apparailly
Pascale Louis-Plence
François Canovas
Pierre Charbord
Danièle Noël
Christian Jorgensen
Publication date
01-04-2007
Publisher
BioMed Central
Published in
Arthritis Research & Therapy / Issue 2/2007
Electronic ISSN: 1478-6362
DOI
https://doi.org/10.1186/ar2153

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