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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 4/2014

01-12-2014 | Translational Research and Biomarkers

Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion

Authors: Kohei Nakata, MD, PhD, Kenoki Ohuchida, MD, PhD, Kazuhiro Mizumoto, MD, PhD, Shinichi Aishima, MD, PhD, Yoshinao Oda, MD, PhD, Eishi Nagai, MD, PhD, Masao Tanaka, MD, PhD, FACS

Published in: Annals of Surgical Oncology | Special Issue 4/2014

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Abstract

Background

Micro RNAs (miRNAs) are small noncoding RNAs that have gained attention as key molecules in the malignant characteristics of cancers, and several recent investigations also have identified some miRNAs as potential key regulators to inhibit the malignant characteristics of tumors. MiRNA-373 (miR-373) has recently been reported to induce E-cadherin, which is a key regulator of epithelial-mesenchymal transition (EMT). However, the role of miR-373 in the characteristics of cancer cells is not still well known.

Methods

We investigated the expression levels of miR-373 in pancreatic cancer cell lines and its effect on the invasiveness of pancreatic cancer by using in vitro and in vivo models. We also analyzed the expression of miR-373 using formalin-fixed paraffin-embedded (n = 152) and microdissected frozen (n = 57) samples from pancreatic tissues.

Results

The levels of miR-373 expression were low in pancreatic cancer cell lines. In formalin-fixed paraffin-embedded and microdissected frozen samples, miR-373 expression was significantly down-regulated in pancreatic cancer compared with that in healthy pancreas (P < 0.001 and P = 0.005, respectively). We also found that reexpression of miR-373 repressed transforming growth factor-β–induced EMT, leading to inhibition of invasiveness of cancer cells. Furthermore, reexpression of miR-373 significantly inhibited peritoneal dissemination in vivo (P < 0.001).

Conclusions

MiR-373 is down-regulated in pancreatic cancer, and its reexpression represses the invasiveness of pancreatic cancer cells.
Appendix
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Metadata
Title
Micro RNA-373 is Down-regulated in Pancreatic Cancer and Inhibits Cancer Cell Invasion
Authors
Kohei Nakata, MD, PhD
Kenoki Ohuchida, MD, PhD
Kazuhiro Mizumoto, MD, PhD
Shinichi Aishima, MD, PhD
Yoshinao Oda, MD, PhD
Eishi Nagai, MD, PhD
Masao Tanaka, MD, PhD, FACS
Publication date
01-12-2014
Publisher
Springer US
Published in
Annals of Surgical Oncology / Issue Special Issue 4/2014
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-014-3676-8

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