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01-12-2019 | Metastasis | Original Article

Enhanced metastatic growth after local tumor resection in the presence of synchronous metastasis in a mouse allograft model of neuroblastoma

Authors: Maho Inoue, Yoshiki Tsuchiya, Nobuya Koike, Yasuhiro Umemura, Hitoshi Inokawa, Yuichi Togashi, Junnosuke Maniwa, Mayumi Higashi, Shigehisa Fumino, Tatsuro Tajiri, Kazuhiro Yagita

Published in: Pediatric Surgery International | Issue 12/2019

Abstract

Purpose

We investigated how local tumor resection affects metastatic lesions in neuroblastoma.

Methods

MYCN Tg tumor-derived cells were injected subcutaneously into 129^+Ter/SvJcl wild-type mice. First, the frequency of metastasis-bearing mice was investigated immunohistochemically (metastatic ratio) at endpoint or post-injection day (PID) 90. Second, the threshold volume of local tumor in mice bearing microscopic lymph node metastasis (mLNM) was investigated at PID 30. Finally, local tumors were resected after exceeding the threshold. Mice were divided into local tumor resection (Resection) and observation (Observation) groups, and the metastatic ratio and volume of LNM were compared between the groups at endpoint or PID 74.

Results

The metastatic ratio without local resection was 88% at PID 78–90. The threshold local tumor volume in the mice with mLNM was 745 mm³ at PID 30, so local tumors were resected after exceeding 700 mm³. The metastatic ratio and LNM volume were significantly greater in the Resection group (n = 16) than in the Observation group (n = 16) (94% vs. 38%, p < 0.001; 2092 ± 2310 vs. 275 ± 218 mm³, p < 0.01; respectively) at PID 50–74.

Conclusion

Local tumor resection might augment the growth of synchronous microscopic metastases. Our results provide insights into the appropriate timing of local resection for high-risk neuroblastoma.

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Title: Enhanced metastatic growth after local tumor resection in the presence of synchronous metastasis in a mouse allograft model of neuroblastoma
Authors: Maho Inoue
Yoshiki Tsuchiya
Nobuya Koike
Yasuhiro Umemura
Hitoshi Inokawa
Yuichi Togashi
Junnosuke Maniwa
Mayumi Higashi
Shigehisa Fumino
Tatsuro Tajiri
Kazuhiro Yagita
Publication date: 01-12-2019
Publisher: Springer Berlin Heidelberg
Keywords: Metastasis
Neuroblastoma
Neuroblastoma
Published in: Pediatric Surgery International / Issue 12/2019
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-019-04568-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Enhanced metastatic growth after local tumor resection in the presence of synchronous metastasis in a mouse allograft model of neuroblastoma

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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