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01-04-2020 | Metastasis | Technical Note

Development of a novel murine model of lymphatic metastasis

Authors: Babak Banan, Jacob A. Beckstead, Lauren E. Dunavant, Yoojin Sohn, Jamie M. Adcock, Sachiyo Nomura, Naji Abumrad, James R. Goldenring, Barbara Fingleton

Published in: Clinical & Experimental Metastasis | Issue 2/2020

Abstract

Current laboratory models of lymphatic metastasis generally require either genetically modified animals or are technically challenging. Herein, we have developed a robust protocol for the induction of intralymphatic metastasis in wild-type mice with reproducible outcomes. To determine an optimal injection quantity and timeline for tumorigenesis, C57Bl/6 mice were injected directly into the mesenteric lymph duct (MLD) with varying numbers of syngeneic murine colon cancer cells (MC38) or gastric cancer cells (YTN16) expressing GFP/luciferase and monitored over 2–4 weeks. Tumor growth was tracked via whole-animal in vivo bioluminescence imaging (IVIS). Our data indicate that the injection of tumor cells into the MLD is a viable model for lymphatic metastasis as necropsies revealed large tumor burdens and metastasis in regional lymph nodes. This protocol enables a closer study of the role of lymphatics in cancer metastasis and opens a window for the development of novel approaches for treatment of metastatic diseases.

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Title: Development of a novel murine model of lymphatic metastasis
Authors: Babak Banan
Jacob A. Beckstead
Lauren E. Dunavant
Yoojin Sohn
Jamie M. Adcock
Sachiyo Nomura
Naji Abumrad
James R. Goldenring
Barbara Fingleton
Publication date: 01-04-2020
Publisher: Springer Netherlands
Keywords: Metastasis
Gastric Cancer
Gastric Cancer
Colon Cancer
Colon Cancer
Published in: Clinical & Experimental Metastasis / Issue 2/2020
Print ISSN: 0262-0898
Electronic ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-020-10025-3

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