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MUSCULOSKELETAL SURGERY

Published in:

01-06-2013 | Original Article

Metalloproteases and their inhibitors are altered in both torn and intact rotator cuff tendons

Authors: A. Castagna, E. Cesari, A. Gigante, M. Conti, R. Garofalo

Published in: MUSCULOSKELETAL SURGERY | Special Issue 1/2013

Abstract

Background

We evaluated the role of matrix metalloproteases (MMPs) and their inhibitors which are involved in extracellular matrix remodeling and degradation, in the pathogenesis of chronic rotator cuff tears.

Materials and Methods

Tendon samples were harvested from 13 patients who underwent arthroscopic repair of a rotator cuff tear. Supraspinatus biopsy specimens were harvested en bloc from the arthroscopically intact middle portion of the tendon more than 1 cm from the torn edge, from the lateral edge of the tear, and from the superior one third of the macroscopically intact subscapularis tendon used as control. Histological analysis and an evaluation of the activity of specific metalloproteases and the tissue inhibitors of metalloprotease (TIMP-1, TIMP-2) was done blindly by multiplex sandwich ELISA (Search-Light technology) in each specimen

Results

Histological evidence of tendinopathy was present in all patients with a tear of the rotator cuff, and not in the macroscopically intact subscapularis tendon. There was a significant increase in MMP 1, MMP 2, MMP 3 and in TIMP-1, TIMP-2 levels in all specimens examined, including the macroscopically intact portion of the supraspinatus tendon and in the control specimens

Conclusions

The tissue in the ruptured area of the supraspinatus tendon undergoes marked rearrangement at molecular levels. This involves the activity of MMP 1, 2 and 3, and supports the critical role of MMPs in the tendon physiology. Seemingly intact parts of the injured supraspinatus tendon can present tendinopathic features, with altered cellular metabolism

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Title: Metalloproteases and their inhibitors are altered in both torn and intact rotator cuff tendons
Authors: A. Castagna
E. Cesari
A. Gigante
M. Conti
R. Garofalo
Publication date: 01-06-2013
Publisher: Springer Milan
Published in: MUSCULOSKELETAL SURGERY / Issue Special Issue 1/2013
Print ISSN: 2035-5106
Electronic ISSN: 2035-5114
DOI: https://doi.org/10.1007/s12306-013-0264-1

At a glance: The STEP trials

Springer Medicine

Metalloproteases and their inhibitors are altered in both torn and intact rotator cuff tendons

Abstract

Background

Materials and Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Materials and Methods

Results

Conclusions

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