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Journal of Gastrointestinal Surgery

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01-01-2011 | 2010 SSAT Poster Presentation

Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum

Authors: Munenori Nagao, David R. Linden, Judith A. Duenes, Michael G. Sarr

Published in: Journal of Gastrointestinal Surgery | Issue 1/2011

Abstract

Aim

This study aims to determine mechanisms of action of the gasotransmitter hydrogen sulfide (H₂S) on contractile activity in longitudinal muscle of rat ileum.

Methods

Ileal longitudinal muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a donor of H₂S, were evaluated on spontaneous contractile activity and after enhanced contractile activity with bethanechol. l-cysteine was evaluated as a potential endogenous donor of H₂S. We evaluated involvement of extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, and K _ATP ⁺ channel and K _Ca ⁺ channel activity on the action of H₂S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-N^G-nitro arginine (l-NNA), glibenclamide, and apamin, respectively, as well as electrical field stimulation.

Result

NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p < 0.05). l-cysteine had no inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NNA, glibenclamide, or apamin had no major effect on total contractile activity by NaHS, although both tetrodotoxin and apamin decreased the frequency of bethanechol-enhanced contractile activity (p < 0.05). We could not demonstrate H₂S release by electrical field stimulation but did show that inhibition of cystathionine β synthase, an endogenous source of H₂S, augmented the inhibitory effect of low-frequency electrical field stimulation.

Conclusion

H₂S inhibits contractile activity of ileal longitudinal muscle dose-dependently but not through pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, K _ATP ⁺ channels, or K _Ca ⁺ channels.

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Title: Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum
Authors: Munenori Nagao
David R. Linden
Judith A. Duenes
Michael G. Sarr
Publication date: 01-01-2011
Publisher: Springer-Verlag
Published in: Journal of Gastrointestinal Surgery / Issue 1/2011
Print ISSN: 1091-255X
Electronic ISSN: 1873-4626
DOI: https://doi.org/10.1007/s11605-010-1306-8

At a glance: The ONWARDS insulin icodec trials

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Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum

Abstract

Aim

Methods

Result

Conclusion

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Abstract

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