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01-10-2012 | Original Article

Mangiferin, a natural polyphenol protects the hepatic damage in mice caused by CCl₄ intoxication

Authors: Mahaboobkhan Rasool, Evan Prince Sabina, P. S. Mahinda, B. Clara Gnanaselvi

Published in: Comparative Clinical Pathology | Issue 5/2012

Abstract

Mangiferin is a natural polyphenolic (C-glucoxyl xanthone) antioxidant present in the bark, fruits, roots and leaves of Mangifera indica Linn. In the present study, we investigated the protective effect of mangiferin against carbon tetrachloride (CCl₄)-induced hepatotoxicity in mice and compared it with silymarin, a standard hepatoprotective drug. The pretreatment of mangiferin (30 mg/kg body weight, i.p.) has shown it to possess a significant protective effect by lowering the serum aspartate and alanine aminotransferases, alkaline phosphatase, bilirubin and inflammatory mediator TNF-α. This hepatoprotective action was confirmed by histological observation. In addition, pretreatment of mangiferin prevented the elevation of hepatic malondialdehyde formation and the depletion of antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and total reduced glutathione) activity in the liver of CCl₄-injected mice. The results suggest that mangiferin exhibits potent hepatoprotective effects on CCl₄-induced liver damages in mice.

Andreu GP, Delgado R, Velho JA, Curti C, Vercesi AE (2005) Iron complexing activity of mangiferin, a naturally occurring glucosylxanthone, inhibits mitochondrial lipid peroxidation induced by Fe(2+)-citrate. Eur J Pharmacol 513:47–55PubMedCrossRef

Bellomo G, Mirabelli F, Dimonte D, Richelmi P, Thor H, Orrenius C, Orrenius S (1987) Formation and reduction of glutathione-mixed disulfides during oxidative stress. Biochem Pharmacol 36:1313–1320PubMedCrossRef

Bishayee A, Sarkar A, Chatterjee M (1995) The hepatoprotective activity of carrot (Daucas carota L.) against carbon tetrachloride intoxication in mouse liver. J Ethnopharmacol 47:69–74PubMedCrossRef

Brautbar N, Williams J (2002) Industrial solvents and liver toxicity: risk assessment, risk factors and mechanisms. Int J Hyg Environ Health 205:479–491PubMedCrossRef

Castro JA, Ferrya GC, Castro CR, Sasame S, Fenes OM, Gilette JR (1974) Prevention of carbon tetrachloride-induced necrosis by inhibitors of drug metabolism. Further studies on the mechanism of their action. Biochem Pharmacol 23:295–302PubMedCrossRef

García D, Delgado R, Ubeira Leiro J (2002) Modulation of rat macrophage function by the Mangifera indica L. extracts and mangiferin. Int Immunopharmacol 2:797–806PubMedCrossRef

Garrido G, Gonzalez D, Lemus Y, Garcia D, Lodeiro L, Quintero G (2004) In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG). Pharmacol Res 50:143–149PubMedCrossRef

Ghosal S, Rao G, Saravanan Misra N, Rana D (1996) A pausible chemical mechanism of the bioactivities of mangiferin. Indian J Chem 35B:561–566

Guha S, Ghosal S, Chattopadhyay U (1996) Antitumor, immunomodulatory and anti-HIV effect of mangiferin, a naturally occurring glucosylxanthone. Chemotherapy 42:443–451PubMedCrossRef

Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139PubMed

Hwang YP, Choi JH, Jeong HG (2009) Protective effect of the Aralia continentalis root extract against carbon tetrachloride-induced hepatotoxicity in mice. Food Chem Toxicol 47:75–81PubMedCrossRef

Leiro JM, Alvarez E, Arranz JA, Siso IG, Orallo F (2003) In vitro effects of mangiferin on superoxide concentrations and expression of the inducible nitric oxide synthase, tumour necrosis factor-alpha and transforming growth factor-beta genes. Biochem Pharmacol 65(8):1361–1371PubMedCrossRef

Lowry OH, Rosebrough NJ, Farr AI, Randall PRJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMed

Marklund SL, Marklund G (1974) Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474PubMedCrossRef

Moron MS, Depierre JW, Mannervik B (1979) Levels of glutathione. glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochem Biophys Acta 582:67–78PubMedCrossRef

Ohkawa H, Ohish N, Yagi K (1997) Assay for lipid peroxides in animal tissues by thiiobarbituric acid. Anal Biochem 95:351–358CrossRef

Pandit S, Sur TK, Jana U, Debnath PK, Sen S, Bhattacharyya D (2004) Prevention of carbon tetrachloride-induced hepatotoxicity in rats by Adhatoda vasica leaves. Indian J Pharmacol 36(5):312–320

Priyadarsini KI, Khopde FM, Kumar SM, Mohan H (2002) Free radical studies of ellagic acid, a natural phenolic antioxidant. J Agric Food Chem 50:2200–2206PubMedCrossRef

Recknagel RO (1983) A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci 33:401–408PubMedCrossRef

Reiter RJ, Tan D, Osuna C, Gitto E (2000) Actions of melatonin in the reduction of oxidative stress. A review. J Biomed Sci 7:444–458PubMedCrossRef

Rotruk JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hekstra WG (1973) Selenium, biochemical role as a component of glutathione peroxidase purification and assay. Science 179:588–590CrossRef

Sanchez GM, Re L, Giuliani A, Nunez-Selles AJ, Davison GP, Leon-Fernandez OS (2000) Protective effects of Mangifera indica L. extract, mangiferin and selected antioxidants against TPA-induced biomolecules oxidation and peritoneal macrophage activation in mice. Pharmacol Res 42:565–573PubMedCrossRef

Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47:389–394PubMedCrossRef

Thyagarajan SP, Jayaram S, Gopalakrishnan V, Hari R, Jeyakumar P, Visioli F, Bellomo G, Galli C (1998) Free radical-scavenging properties of olive oil polyphenols. Biochem Biophys Res Commun 247:60–64CrossRef

Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84PubMedCrossRef

Venukumar MR, Latha MS (2002) Antioxidant activity of Curculigo orchioides in carbon tetrachloride-induced hepatopathy in rats. Indian J Clin Biochem 17:80–87CrossRef

Wang BJ, Liu CT, Tseng CY, Wu CP, Yu ZR (2004) Hepatoprotective and antioxidant effects of Bupleurum kaoi Liu (Chao et Chuang) extract and its fractions fractionated using supercritical CO2 on CCl4-induced liver damage. Food Chem Toxicol 42:609–617PubMedCrossRef

Weber LWD, Boll M, Stampfl A (2003) Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Crit Rev Toxicol 33:105–136PubMedCrossRef

Yadav NP, Dixit VK (2003) Hepatoprotective activity of leaves of Kalanchoe pinnata Pers. J Ethnopharmacol 86(23):197–202PubMedCrossRef

Youssef W, McCullough AJ (2002) Diabetes mellitus, obesity, and hepatic steatosis. Semin Gastrointest Dis 13:17–30PubMed

Zheng MS, Lu ZY (1990) Antiviral effect of mangiferin and isomangiferin on herpes simplex virus. Chin Med J 103:160–165PubMed

Title: Mangiferin, a natural polyphenol protects the hepatic damage in mice caused by CCl4 intoxication
Authors: Mahaboobkhan Rasool
Evan Prince Sabina
P. S. Mahinda
B. Clara Gnanaselvi
Publication date: 01-10-2012
Publisher: Springer-Verlag
Published in: Comparative Clinical Pathology / Issue 5/2012
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-011-1190-y

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Mangiferin, a natural polyphenol protects the hepatic damage in mice caused by CCl₄ intoxication

Abstract

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