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Tumor Biology
Published in: Tumor Biology 4/2011

Open Access 01-08-2011 | Research Article

Major regulators of microRNAs biogenesis Dicer and Drosha are down-regulated in endometrial cancer

Authors: Anna Torres, Kamil Torres, Tomasz Paszkowski, Barbara Jodłowska-Jędrych, Tomasz Radomański, Andrzej Książek, Ryszard Maciejewski

Published in: Tumor Biology | Issue 4/2011

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Abstract

Alterations in microRNAs expression have been proposed to play role in endometrial cancer pathogenesis. Dicer and Drosha are main regulators of microRNA biogenesis and deregulation of their expression has been indicated as a possible cause of microRNAs alterations observed in various cancers. The objective of this study was to investigate Dicer and Drosha genes expression in endometrial cancer and to analyze the impact of clinicopathological characteristics on their expression. Fresh tissue samples were collected from 44 patients (26 endometroid endometrial carcinoma and 18 controls). Clinical and pathological data were acquired from medical documentation. Dicer and Drosha genes expressions were assessed by qRT-PCR using validated reference genes. Dicer and Drosha expression levels were significantly lower in endometrial cancer samples comparing to controls. Dicer was down-regulated by the factor of 1.54 (p = 0.009) and Drosha gene mean expression value was 1.4 times lower in endometrial cancer group versus control group (p = 0.008). Down-regulation of Dicer significantly correlated with decreased expression of Drosha (coefficient value 0.75). Decreased expression of Drosha correlated with higher histological grade and was influenced by BMI. Lower Dicer expression was found in nulli- and uniparous females comparing to multiparous individuals (p = 0.002). Neither the FIGO stage nor the menstrual status had significant influence on the expression of studied genes. This study revealed for the first time that expression alterations of main regulators of microRNAs biogenesis are present in endometrial cancer tissue and could be potentially responsible for altered microRNAs profiles observed in this malignancy.
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Metadata
Title
Major regulators of microRNAs biogenesis Dicer and Drosha are down-regulated in endometrial cancer
Authors
Anna Torres
Kamil Torres
Tomasz Paszkowski
Barbara Jodłowska-Jędrych
Tomasz Radomański
Andrzej Książek
Ryszard Maciejewski
Publication date
01-08-2011
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 4/2011
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-011-0179-0

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