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01-06-2009 | Original Article

Lupus antibodies to the HMGB1 chromosomal protein: epitope mapping and association with disease activity

Authors: Ayako Hayashi, Hiroko Nagafuchi, Ichiaki Ito, Koichi Hirota, Michiteru Yoshida, Shoichi Ozaki

Published in: Modern Rheumatology | Issue 3/2009

Abstract

The high mobility group box 1 (HMGB1) protein is a non-histone chromosomal protein that acts as a potent proinflammatory cytokine when actively secreted from LPS- or TNF-activated macrophages, monocytes, and other cells. Anti-HMGB1/2 antibodies have been previously identified in sera from a high proportion of patients with autoimmune diseases. In this study, we examined anti-HMGB1 antibody titers in sera of patients with systemic rheumatic diseases and the correlations between the presence of anti-HMGB1 antibodies and disease activity in systemic lupus erythematosus (SLE) patients by enzyme-linked immunosorbent assay and western blotting. We detected increases in both the levels and the frequency of anti-HMGB1 antibodies in sera from SLE and polymyositis/dermatomyositis (PM/DM) patients, and observed that the presence of anti-HMGB1 antibodies positively correlates with SLE disease activity index. Through epitope mapping, we found that multiple HMGB1 epitopes were recognised in SLE sera, with the major epitope mapping to box A. Another epitope, the joiner region of HMGB1, was preferentially recognized by SLE sera, but not by PM/DM sera. Collectively, these observations suggest that the presence of anti-HMGB1 antibodies correlates with disease activity in SLE patients.

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Title: Lupus antibodies to the HMGB1 chromosomal protein: epitope mapping and association with disease activity
Authors: Ayako Hayashi
Hiroko Nagafuchi
Ichiaki Ito
Koichi Hirota
Michiteru Yoshida
Shoichi Ozaki
Publication date: 01-06-2009
Publisher: Springer Japan
Published in: Modern Rheumatology / Issue 3/2009
Print ISSN: 1439-7595
Electronic ISSN: 1439-7609
DOI: https://doi.org/10.1007/s10165-009-0151-7

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