Top

Published in:

01-02-2013 | IM - ORIGINAL

Low frequency of cagA-positive Helicobacter pylori strains isolated from Iranian patients with MALT lymphoma

Authors: Amin Talebi Bezmin Abadi, Ali Ghasemzadeh, Ashraf Mohabati Mobarez

Published in: Internal and Emergency Medicine | Issue 1/2013

Abstract

Helicobacter pylori is predominantly involved in the etiology of digestive diseases. The aim of our study is to determine the relationship of cagA frequency with less investigated gastroduodenal disorders such as MALT (mucosal associated lymphoid tissue) lymphoma and gastric cancer. One hundred-twenty eight H. pylori-positive patients including: gastritis (n = 74), gastric cancer (n = 26) and MALT lymphoma (n = 28) were entered in our study. Antral biopsy specimen transport, bacterial culture and cagA detection were performed based on standard protocols. In brief, biopsies from positive H. pylori patients were investigated for presence of cagA gene by polymerase chain reactions (PCR) method. Of 128 consecutive Iranian patients with gastroduodenal disorders examined in our study, we identified 84 (65.6%) cagA-positive strains. However, six patients were excluded because of negative culture for identification of H. pylori. Prevalence of cagA in each categorized groups are following: 63/74 (85.1%) of gastritis patients, 16/28 (57.1%) and 5/26 gastric cancer (19.2%) of MALT lymphoma, respectively. Current findings reveal that the presence of cagA is not a reliable marker for prediction of digestive disorders caused by H. pylori infection. All our patients with gastric cancer were diagnosed as adenocarcinoma. The low rate of cagA among gastric cancer and MALT lymphoma groups was not statistically significant, possibly due to the small number of patients enrolled in the study. We suggest that a study with a high number of patients is needed for making more definitive assessment of the correlation between cagA-positive H. pylori and gastric cancer and MALT lymphoma.

Bornschein J, Rokkas T, Selgrad M, Malfertheiner P (2009) Helicobacter pylori and clinical aspects of gastric cancer. Helicobacter 14:41–45PubMedCrossRef

Peek RM, Thompson SA, Donahue JP, Tham KT, Atherton JC, Blaser MJ, Miller GG (1998) Adherence to gastric epithelial cells induces expression of a Helicobacter pylori gene, iceA that is associated with clinical outcome. Proc Assoc Am Physicians 110:531–544PubMed

Parsonnet J (1995) The incidence of Helicobacter pylori infection. Aliment Pharmacol Ther 9:45–51PubMed

Kusters JG, Vliet HM, Kuipers EJ (2006) Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 19:449–490PubMedCrossRef

Audibert C, Burucoa C, Janvier B, Fauchere JL (2001) Implication of the structure of the Helicobacter pylori cag pathogenicity island in induction of interleukin-8 secretion. Infect Immun 69:1625–1629PubMedCrossRef

Salehi Z, Jelodar MH, Rassa M, Ahaki M, Mollasalehi H, Mashayekhi F (2009) Helicobacterpylori cagA status and peptic ulcer disease in Iran. Dig Dis Sci 54:608–613PubMedCrossRef

Kersulyte D, Mukhopadhyay A, Velapatin B, Su W et al (2000) Differences in genotypes of Helicobacter pylori from different human populations. J Bacteriol 182:3210–3218PubMedCrossRef

Amsterdam K, Van Vliet AHM, Kusters JG, Der Ende A (2006) Of microbe and man: determinants of Helicobacter pylori-related diseases. FEMS Microbiol Rev 30:131–156PubMedCrossRef

Oliveira MJ, Costa AC, Costa AM, Henriques L, Suriano G, Atherton JC et al (2006) Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system dependent manner. Biol Chem 46:34888–34896

10.

Costa AA, Figueiredo C, Touati E (2009) Pathogenesis of Helicobacter pylori infection. Helicobacter 14:15–20PubMedCrossRef

11.

Huang J, Toole PW, Doig P, Trust TJ (1995) Stimulation of interleukin-8 in epithelial cell line by Helicobacter pylori. Infect Immun 63:1732–1738PubMed

12.

Queiroz DMM, Mendes EM, Rocha GA et al (1998) cagA positive Helicobacter pylori and risk for developing gastric carcinoma in Brazil. Int J Cancer 78:135–139PubMedCrossRef

13.

Yamazaki S, Yamakawa A, Okuda T, Ohtani M et al (2005) Distinct diversity of vacA, cagA, and cagE Genes of Helicobacter pylori associated with peptic ulcer in Japan. J Clin Microbiol 45:3906–3916CrossRef

14.

Andreson H, Lõivukene K, Sillakivi T et al (2002) Association of cagA and vacA Genotypes of Helicobacter pylori with gastric diseases in Estonia. J Clin Microbiol 40:298–300PubMedCrossRef

15.

Eidt S, Stolte M, Fischer R (1994) Helicobacter pylori gastritis and primary gastric non-Hodgkin’s lymphomas. J Clin Pathol. 47:436–439PubMedCrossRef

16.

Parsonnet J, Hansen S, Rodriguez L, Gelb AB, Warnke RA, Jellum E et al (1994) Helicobacter pylori infection and gastric lymphoma. N Engl J Med 330:1267–1271PubMedCrossRef

17.

Figueiredo C, van Doorn LJ, Nogueira C, Soares JM, Pinho C, Figueira P, Quint WG, Carneiro F (2001) Helicobacter pylori genotypes are associated with clinical outcome in Portuguese patients and show a high prevalence of infections with multiple strains. Scand J Gastroenterol 36:128–135PubMedCrossRef

18.

Miehlike S, Schuppler M, Frings C et al (2001) Helicobacter pylori vacA, iceA and cagA status and pattern of gastritis in patients with malignant and benign gastroduodenal disease. Am J Gastroentrol 96:1008–1013CrossRef

19.

Schumann C, Triantafilou K, Rasche FM, Möricke A, Vogt K, Triantafilou M, Hahn P, Schneider EM, Lepper PM (2006) Serum antibody positivity for distinct Helicobacter pylori antigens in benign and malignant gastroduodenal disease. Int J Med Microbiol 296:223–228PubMedCrossRef

20.

Jaber SM (2005) The pattern of CagA and VacA proteins in Helicobacter pylori seropositive asymptomatic children in western Saudi Arabia. Saudi Med J 26:1372–1377PubMed

21.

Mattar R, Barbosa Marques S, Do Socorro Monteiro M et al (2007) Helicobacter pylori cag pathogenicity island genes: clinical relevance for peptic ulcer disease development in Brazil. J Med Microbiol 56:9–14PubMedCrossRef

22.

Malekzadeh R, Derakhshan MH, Malekzadeh Z (2009) Gastric cancer in Iran: epidemiology and risk factors. Arch Iran Med 12:576–583PubMed

23.

Nouraie M, Latifi-Navid S, Rezvan H, Radmard AR, Maghsudlu M, Zaer-Rezaii H, Amini S, Siavoshi F, Malekzadeh R (2009) Childhood hygienic practice and family education status determine the prevalence of H. pylori infection in Iran. Helicobacter 14:40–46PubMedCrossRef

24.

Jafarzadeh A, Ahmedi-Kahanali J, Bahrami M, Taghipour Z (2007) Seroprevalence of anti-Helicobacter pylori and anti-CagA antibodies among healthy children according to age, sex, ABO blood groups and Rh status in south-east of Iran. Turk J Gastroenterol 18:165–171PubMed

25.

Bazargani A, Ekrami A, Bassiri E, Saber Firoozi M (2005) Frequency of CagA in Helicobacter Pylori isolates of patients with peptic ulcer diseases (PUD) and nonulcer dyspepsia (NUD) at Namazi Hospital, Shiraz, Iran. Govaresh 10:116–119

26.

Tiwari SK, Khan AA, Ahmed KS, Ali SM, Ahmed I, Habeeb A et al (2005) Polymerase chain reaction based analysis of the Cag PAI of Helicobacter pylori from saliva. J Gastroenterol Hepatol 20:1560–1566PubMedCrossRef

27.

Safaei H, Tavakkoli H, Ali Mojtahedi A, Salehei R, Soleimani B, Pishva E (2008) Correlation of cagA positive Helicobacter pylori Infection with clinical outcomes in Alzahra Hospital, Isfahan, Iran. JRMS 13:196–201

28.

Erzin Y, Koksal V, Altun S, Dobrucali A, Aslan M, Erdamar S, Dirican A, Kocazeybek B (2006) Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA, babA ₂ genotypes and correlation with clinical outcome in Turkish patients with dyspepsia. Helicobacter 11:574–580PubMedCrossRef

29.

Douraghi M, Mohammadi M, Shirazi MH et al (2009) Simultaneous detection of cagA and cagE of Helicobacter pylori strains recovered from Iranian patients with different gastroduodenal diseases. Iranian J Publ Health 38:98–105

30.

Douraghi M, Mohammadi M, Shirazi MH, Maryam E, Bababeyk M, Saberi Kashani S, Oghalaei A, Mohajerani N (2008) Assessment the relationship of cagA gene with different gastroduodenal diseases in Helicobacter pylori infected patients. Iranian J Med Microbiol 2:31–36

31.

Dabiri H, Maleknejad P, Yamaoka Y, Feizabadi MM, Jafari F, Rezadehbashi M et al (2009) Distribution of Helicobacter pylori cagA, cagE, oipA and vacA in different major ethnic groups in Tehran, Iran. J Gastroenterol Hepatol 24:1380–1386PubMedCrossRef

32.

Paniagua GL, Monroy E, Rodriguez R, Arroniz S, Rodriguez C, Cortes J et al (2009) Frequency of vacA, cagA and babA ₂ virulence markers in Helicobacter pylori strains isolated from Mexican patients with chronic gastritis. Ann Clin Microbiol Antimicrob 30:8–14. doi:10.1186/1476-0711-8-14

33.

Oleastro M, Gerhard M, Lopes AI, Ramalho P et al (2003) Helicobacter pylori virulence genotypes in Portuguese children and adults with gastroduodenal pathology. Eur J Clin Microbiol Infect Dis. 22:85–91PubMed

34.

Torres LE, Melian K, Moreno A, Alonso J, Sabatier CA, Hernandez M, Bermudez L, Rodriguez BL (2009) Prevalence of vacA, cagA and babA ₂ genes in Cuban Helicobacter pylori isolates. World J Gastroenterol 14:204–210CrossRef

35.

Warburton VJ, Everett S, Mapstone NP, Axon AT et al (1998) Clinical and histological associations of cagA and vacA genotypes in Helicobacter pylori gastritis. J Clin Pathol 51:55–61PubMedCrossRef

36.

Talebkhan Y, Mohammadi M, Mohagheghi MA, Vaziri HR et al (2008) cagA gene and protein status among Iranian Helicobacter pylori strains. Dig Dis Sci 53:925–932PubMedCrossRef

37.

Yakoob J, Fan XG, Peng XN, Hu GL, Zhang Z (2002) Helicobacter pylori cag A and vacA cytotoxin genes in Changsha, China. Br J Biomed Sci 59:150–153PubMed

38.

Lin HJ, Perng CL, Lo WC, Wu CW et al (2004) Helicobacter pylori cagA, iceA and vacA genotypes in patients with gastric cancer in Taiwan. World J Gastroenterol l10:2493–2497

39.

Jafari F, Shokrzadeh L, Dabiri H, Baghaei K, Yamaoka Y, Zojaji H, Haghazali M, Molaei M, Zali MR (2008) vacA genotypes of Helicobacter pylori in relation to cagA status and clinical outcomes in Iranian populations. Jpn J Infect Dis 6:290–293

40.

Talebi Bezmin Abadi A, Mohabati Mobarez A, Taghvaei T, Wolfram L (2010) Antibiotic resistance of Helicobacter pylori in Mazandaran, North of Iran. Helicobacter 15:505–509PubMedCrossRef

41.

Chomvarin C, Namwat W, Chaicumpar K, Mairiang P, Sangchan A et al (2008) Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients. Int J Infect Dis 12(1):30–36

Title: Low frequency of cagA-positive Helicobacter pylori strains isolated from Iranian patients with MALT lymphoma
Authors: Amin Talebi Bezmin Abadi
Ali Ghasemzadeh
Ashraf Mohabati Mobarez
Publication date: 01-02-2013
Publisher: Springer Milan
Published in: Internal and Emergency Medicine / Issue 1/2013
Print ISSN: 1828-0447
Electronic ISSN: 1970-9366
DOI: https://doi.org/10.1007/s11739-011-0579-6

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2013

News for 2013

Lung ultrasound imaging in haemodialysis

Evaluation of the current prognostic role of cardiogenic syncope

An unusual syncope

Serum 25-hydroxyvitamin D levels are inversely associated with systemic inflammation in severe obese subjects

Palpebral ptosis, fixed mydriasis and exophthalmos: an uncommon presentation of new diabetes mellitus

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials