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BMC Immunology
Published in: BMC Immunology 1/2012

Open Access 01-12-2012 | Research article

Low-dose plasmid DNA treatment increases plasma vasopressin and regulates blood pressure in experimental endotoxemia

Authors: Thiago Malardo, Marcelo E Batalhão, Ademilson Panunto-Castelo, Luciana P Almeida, Everton Padilha, Isabela C Fontoura, Célio L Silva, Evelin C Carnio, Arlete AM Coelho-Castelo

Published in: BMC Immunology | Issue 1/2012

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Abstract

Background

Although plasmid DNA encoding an antigen from pathogens or tumor cells has been widely studied as vaccine, the use of plasmid vector (without insert) as therapeutic agent requires further investigation.

Results

Here, we showed that plasmid DNA (pcDNA3) at low doses inhibits the production of IL-6 and TNF-α by lipopolysaccharide (LPS)-stimulated macrophage cell line J774. These findings led us to evaluate whether plasmid DNA could act as an anti-inflammatory agent in a Wistar rat endotoxemia model. Rats injected simultaneously with 1.5 mg/kg of LPS and 10 or 20 μg of plasmid DNA had a remarkable attenuation of mean arterial blood pressure (MAP) drop at 2 hours after treatment when compared with rats injected with LPS only. The beneficial effect of the plasmid DNA on MAP was associated with decreased expression of IL-6 in liver and increased concentration of plasma vasopressin (AVP), a known vasoconstrictor that has been investigated in hemorrhagic shock management. No difference was observed in relation to nitric oxide (NO) production.

Conclusion

Our results demonstrate for the first time that plasmid DNA vector at low doses presents anti-inflammatory property and constitutes a novel approach with therapeutic potential in inflammatory diseases.
Appendix
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Metadata
Title
Low-dose plasmid DNA treatment increases plasma vasopressin and regulates blood pressure in experimental endotoxemia
Authors
Thiago Malardo
Marcelo E Batalhão
Ademilson Panunto-Castelo
Luciana P Almeida
Everton Padilha
Isabela C Fontoura
Célio L Silva
Evelin C Carnio
Arlete AM Coelho-Castelo
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Immunology / Issue 1/2012
Electronic ISSN: 1471-2172
DOI
https://doi.org/10.1186/1471-2172-13-59

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