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Open Access 01-12-2010 | Research article

Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma

Authors: Rene' G Feichtinger, Franz Zimmermann, Johannes A Mayr, Daniel Neureiter, Cornelia Hauser-Kronberger, Freimut H Schilling, Neil Jones, Wolfgang Sperl, Barbara Kofler

Published in: BMC Cancer | Issue 1/2010

Abstract

Background

Succinate dehydrogenase (SDH) has been associated with carcinogenesis in pheochromocytoma and paraganglioma. In the present study we investigated components of the oxidative phosphorylation system in human neuroblastoma tissue samples.

Methods

Spectrophotometric measurements, immunohistochemical analysis and Western blot analysis were used to characterize the aerobic mitochondrial energy metabolism in neuroblastomas (NB).

Results

Compared to mitochondrial citrate synthase, SDH activity was severely reduced in NB (n = 14) versus kidney tissue. However no pathogenic mutations could be identified in any of the four subunits of SDH. Furthermore, no genetic alterations could be identified in the two novel SDH assembly factors SDHAF1 and SDH5. Alterations in genes encoding nfs-1, frataxin and isd-11 that could lead to a diminished SDH activity have not been detected in NB.

Conclusion

Because downregulation of other complexes of the oxidative phosphorylation system was also observed, a more generalized reduction of mitochondrial respiration seems to be present in neuroblastoma in contrast to the single enzyme defect found in hereditary pheochromocytomas.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/10/149/prepub

Title: Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma
Authors: Rene' G Feichtinger
Franz Zimmermann
Johannes A Mayr
Daniel Neureiter
Cornelia Hauser-Kronberger
Freimut H Schilling
Neil Jones
Wolfgang Sperl
Barbara Kofler
Publication date: 01-12-2010
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2010
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-10-149

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Background

Methods

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