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Clinical Orthopaedics and Related Research®

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01-07-2008 | Symposium: Molecular and Clinical Developments in Tendinopathy

Loss of Homeostatic Strain Alters Mechanostat “Set Point” of Tendon Cells In Vitro

Authors: Steven P. Arnoczky, DVM, Michael Lavagnino, PhD, Monika Egerbacher, DVM, PhD, Oscar Caballero, MS, Keri Gardner, MS, Marisa A. Shender

Published in: Clinical Orthopaedics and Related Research® | Issue 7/2008

Abstract

Tendon cells respond to mechanical loads. The character (anabolic or catabolic) and sensitivity of this response is determined by the mechanostat set point of the cell, which is governed by the cytoskeleton and its interaction with the extracellular matrix. To determine if loss of cytoskeletal tension following stress deprivation decreases the mechanoresponsiveness of tendon cells, we cultured rat tail tendons under stress-deprived conditions for 48 hours and then cyclically loaded them for 24 hours at 1%, 3%, or 6% strain at 0.17 Hz. Stress deprivation upregulated MMP-13 mRNA expression and caused progressive loss of cell-matrix contact compared to fresh controls. The application of 1% strain to fresh tendons for 24 hours inhibited MMP-13 mRNA expression compared to stress-deprived tendons over the same period. However, when tendons were stress-deprived for 48 hours and then subjected to the same loading regime, the inhibition of MMP-13 mRNA expression was decreased. In stress-deprived tendons, it was necessary to increase the strain magnitude to 3% to achieve the same level of MMP-13 mRNA inhibition seen in fresh tendons exercised at 1% strain. The data suggest loss of cytoskeletal tension alters the mechanostat set point and decreases the mechanoresponsiveness of tendon cells.

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Title: Loss of Homeostatic Strain Alters Mechanostat “Set Point” of Tendon Cells In Vitro
Authors: Steven P. Arnoczky, DVM
Michael Lavagnino, PhD
Monika Egerbacher, DVM, PhD
Oscar Caballero, MS
Keri Gardner, MS
Marisa A. Shender
Publication date: 01-07-2008
Publisher: Springer-Verlag
Published in: Clinical Orthopaedics and Related Research® / Issue 7/2008
Print ISSN: 0009-921X
Electronic ISSN: 1528-1132
DOI: https://doi.org/10.1007/s11999-008-0264-x

At a glance: The STEP trials

Springer Medicine

Loss of Homeostatic Strain Alters Mechanostat “Set Point” of Tendon Cells In Vitro

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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