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01-01-2024 | Brief Report

Long-term passages of Plantago asiatica mosaic virus alter virulence and multiplication in Nicotiana benthamiana plants

Authors: Daisuke Nakamura, Nami Minato, Minako Furuya, Ken Komatsu, Shin-ichi Fuji

Published in: Archives of Virology | Issue 1/2024

Abstract

We demonstrated the infectivity and host adaptation of a viola isolate of Plantago asiatica mosaic virus (PlAMV-Vi) in an asymptomatic host, Nicotiana benthamiana, through long-term serial passages. Serial passaging of a green fluorescent protein–tagged full-length cDNA clone of PlAMV-Vi (PlAMV-Vi_GFP) in N. benthamiana plants resulted in the appearance of a new virus line inducing leaf-crinkle symptoms, the Leaf Crinkle (LC) line. Virus titers were higher for both in the LC and the 14th passage line(s) of PlAMV-Vi_GFP compared with the original line. The LC line was found to have seven unique nucleotide mutations that may have contributed to its higher virulence and multiplication rate in N. benthamiana.

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Title: Long-term passages of Plantago asiatica mosaic virus alter virulence and multiplication in Nicotiana benthamiana plants
Authors: Daisuke Nakamura
Nami Minato
Minako Furuya
Ken Komatsu
Shin-ichi Fuji
Publication date: 01-01-2024
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 1/2024
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-023-05934-z

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